دورية أكاديمية
The p38-MITOGEN-ACTIVATED PROTEIN KINASE Signaling Pathway Is Involved in Leonurus artemisia Extract-Induced Inhibition of the Proliferation of Human Bladder Cancer BFTC-905 Cells via G1/G0 Arrest and Causes Apoptosis In Vitro
العنوان: | The p38-MITOGEN-ACTIVATED PROTEIN KINASE Signaling Pathway Is Involved in Leonurus artemisia Extract-Induced Inhibition of the Proliferation of Human Bladder Cancer BFTC-905 Cells via G1/G0 Arrest and Causes Apoptosis In Vitro |
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المؤلفون: | Jian-Hui Lin, Chein-Hui Hung, Yun-Ching Huang, Chih-Shou Chen, Dong-Ru Ho |
المصدر: | Pharmaceuticals, Vol 16, Iss 10, p 1338 (2023) |
بيانات النشر: | MDPI AG, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Medicine LCC:Pharmacy and materia medica |
مصطلحات موضوعية: | Leonurus artemisia extract, Chinese motherwort, Scientific Chinese Medicine extract, bladder cancer, BFTC-905 cells, reactive oxygen species, Medicine, Pharmacy and materia medica, RS1-441 |
الوصف: | Bladder cancer is a urothelial malignancy. Bladder cancer starts in the urothelial cells lining the inside of the bladder. The 5-year recurrence rate for bladder cancer ranges from 31% to 78%, and the progression rate is approximately 45%. To treat bladder cancer, intravesical drug therapy is often used. Leonurus artemisia extract (LaE) was obtained from medicinal samples of Chinese motherwort Scientific Chinese Medicine; L. artemisia has various biological effects. This study investigated the impact of LaE on human bladder cancer cells (the BFTC-905 cell line) and the molecular mechanism underlying apoptosis resulting from the activation of cell signal transduction pathways in bladder cancer cells. A cell counting kit-8 (CCK-8) assay was used to determine the effect of LaE on cell growth. The effect of LaE on migration ability was observed using a wound healing assay. The effects of LaE on the cell cycle, reactive oxygen species production, and apoptosis were investigated. Western blot analysis detected apoptosis-related and mitogen-activated protein kinase signaling pathway-related protein concentrations. At non-toxic concentrations, LaE inhibited the proliferation of BFTC-905 cells in a concentration-dependent manner, and the half-maximal inhibitory concentration (IC50) was 24.08172 µg/µL. LaE impaired the migration ability of BFTC-905 cells. LaE arrested the cell cycle in the G1 and G0 phases, increased reactive oxygen species production, and induced apoptosis. LaE increased Bax and p-ERK concentrations and decreased Bcl-2, cleaved caspase-3, and p-p38 concentrations. No differences in PARP, C-PARP, vimentin, e-cadherin, p-JNK, or TNF-alpha concentrations were observed. These results suggest that LaE inhibits the proliferation of human bladder cancer cells. Moreover, the mitogen-activated protein kinase signaling pathway is involved in the inhibition of the proliferation of BFTC-905 cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1424-8247 |
Relation: | https://www.mdpi.com/1424-8247/16/10/1338; https://doaj.org/toc/1424-8247 |
DOI: | 10.3390/ph16101338 |
URL الوصول: | https://doaj.org/article/d896ca350fb0425dbc6a40934f9ba9ab |
رقم الأكسشن: | edsdoj.896ca350fb0425dbc6a40934f9ba9ab |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14248247 |
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DOI: | 10.3390/ph16101338 |