دورية أكاديمية
Ziziphus jujuba Miller Ethanol Extract Restores Disrupted Intestinal Barrier Function via Tight Junction Recovery and Reduces Inflammation
| العنوان: | Ziziphus jujuba Miller Ethanol Extract Restores Disrupted Intestinal Barrier Function via Tight Junction Recovery and Reduces Inflammation |
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| المؤلفون: | Ye Jin Yang, Min Jung Kim, Ho Jeong Lee, Won-Yung Lee, Ju-Hye Yang, Hun Hwan Kim, Min Sup Shim, Ji Woong Heo, Jae Dong Son, Woo H. Kim, Gon Sup Kim, Hu-Jang Lee, Young-Woo Kim, Kwang Youn Kim, Kwang Il Park |
| المصدر: | Antioxidants, Vol 13, Iss 5, p 575 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Ziziphus jujuba Miller, anti-inflammation, intestinal barrier function, tight junction, inflammatory bowel disease, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Inflammatory bowel disease (IBD) is a chronic inflammatory condition caused by the disruption of the intestinal barrier. The intestinal barrier is maintained by tight junctions (TJs), which sustain intestinal homeostasis and prevent pathogens from entering the microbiome and mucosal tissues. Ziziphus jujuba Miller (Z. jujuba) is a natural substance that has been used in traditional medicine as a therapy for a variety of diseases. However, in IBD, the efficacy of Z. jujuba is unknown. Therefore, we evaluated ZJB in Caco2 cells and a dextran sodium sulfate (DSS)-induced mouse model to demonstrate its efficacy in IBD. Z. jujuba extracts were prepared using 70% ethanol and were named ZJB. ZJB was found to be non-cytotoxic and to have excellent antioxidant effects. We confirmed its anti-inflammatory properties via the down-regulation of inflammatory factors, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). To evaluate the effects of ZJB on intestinal barrier function and TJ improvement, the trans-epithelial electrical resistance (TEER) and fluorescein isothiocyanate-dextran 4 kDa (FITC-Dextran 4) permeability were assessed. The TEER value increased by 61.389% and permeability decreased by 27.348% in the 200 μg/mL ZJB group compared with the 50 ng/mL IL-6 group after 24 h. Additionally, ZJB alleviated body weight loss, reduced the disease activity index (DAI) score, and induced colon shortening in 5% DSS-induced mice; inflammatory cytokines, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were down-regulated in the serum. TJ proteins, such as Zonula occludens (ZO)-1 and occludin, were up-regulated by ZJB in an impaired Caco2 mouse model. Additionally, according to the liquid chromatography results, in tandem with mass spectrometry (LC-MS/MS) analysis, seven active ingredients were detected in ZJB. In conclusion, ZJB down-regulated inflammatory factors, protected intestinal barrier function, and increased TJ proteins. It is thus a safe, natural substance with the potential to be used as a therapeutic agent in IBD treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 13050575 2076-3921 |
| Relation: | https://www.mdpi.com/2076-3921/13/5/575; https://doaj.org/toc/2076-3921 |
| DOI: | 10.3390/antiox13050575 |
| URL الوصول: | https://doaj.org/article/8974db0295cc42b6a1b137316f6acf41 |
| رقم الأكسشن: | edsdoj.8974db0295cc42b6a1b137316f6acf41 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13050575 20763921 |
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| DOI: | 10.3390/antiox13050575 |