دورية أكاديمية
Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase
| العنوان: | Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase |
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| المؤلفون: | Sanaz Ahmadipour, Alice J. C. Wahart, Jonathan P. Dolan, Laura Beswick, Chris S. Hawes, Robert A. Field, Gavin J. Miller |
| المصدر: | Beilstein Journal of Organic Chemistry, Vol 18, Iss 1, Pp 1379-1384 (2022) |
| بيانات النشر: | Beilstein-Institut, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science LCC:Organic chemistry |
| مصطلحات موضوعية: | alginate, chemical probe, enzymatic synthesis, gdp-mannose dehydrogenase, sugar nucleotide, Science, Organic chemistry, QD241-441 |
| الوصف: | Sufferers of cystic fibrosis are at significant risk of contracting chronic bacterial lung infections. The dominant pathogen in these cases is mucoid Pseudomonas aeruginosa. Such infections are characterised by overproduction of the exopolysaccharide alginate. We present herein the design and chemoenzymatic synthesis of sugar nucleotide tools to probe a critical enzyme within alginate biosynthesis, GDP-mannose dehydrogenase (GMD). We first synthesise C6-modified glycosyl 1-phosphates, incorporating 6-amino, 6-chloro and 6-sulfhydryl groups, followed by their evaluation as substrates for enzymatic pyrophosphorylative coupling. The development of this methodology enables access to GDP 6-chloro-6-deoxy-ᴅ-mannose and its evaluation against GMD. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1860-5397 |
| Relation: | https://doaj.org/toc/1860-5397 |
| DOI: | 10.3762/bjoc.18.142 |
| URL الوصول: | https://doaj.org/article/897c1b0ffb0340a0afbd2a9a48f64934 |
| رقم الأكسشن: | edsdoj.897c1b0ffb0340a0afbd2a9a48f64934 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18605397 |
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| DOI: | 10.3762/bjoc.18.142 |