دورية أكاديمية
Cell lineage-specific genome-wide DNA methylation analysis of patients with paediatric-onset systemic lupus erythematosus
| العنوان: | Cell lineage-specific genome-wide DNA methylation analysis of patients with paediatric-onset systemic lupus erythematosus |
|---|---|
| المؤلفون: | Kit San Yeung, Tsz Leung Lee, Mo Yin Mok, Christopher Chun Yu Mak, Wanling Yang, Patrick Chun Yin Chong, Pamela Pui Wah Lee, Marco Hok Kung Ho, Sanaa Choufani, Chak Sing Lau, Yu Lung Lau, Rosanna Weksberg, Brian Hon Yin Chung |
| المصدر: | Epigenetics, Vol 14, Iss 4, Pp 341-351 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | sle, lupus, paediatric-onset, dna methylation, methylationepic, blood, cell lineage-specific, cell composition, Genetics, QH426-470 |
| الوصف: | Patients with paediatric-onset systemic lupus erythematosus (SLE) often present with more severe clinical courses than adult-onset patients. Although genome-wide DNA methylation (DNAm) profiling has been performed in adult-onset SLE patients, parallel data on paediatric-onset SLE are not available. Therefore, we undertook a genome-wide DNAm study in paediatric-onset SLE patients across multiple blood cell lineages. The DNAm profiles of four purified immune cell lineages (CD4 + T cells, CD8 + T cells, B cells and neutrophils) and whole blood were compared in 16 Chinese patients with paediatric-onset SLE and 13 healthy controls using the Illumina HumanMethylationEPIC BeadChip. Comparison of DNAm in whole blood and within each independent cell lineage identified a consistent pattern of loss of DNAm at 21 CpG sites overlapping 15 genes, which represented a robust, disease-specific DNAm signature for paediatric-onset SLE in our cohort. In addition, cell lineage-specific changes, involving both loss and gain of DNAm, were observed in both novel genes and genes with well-described roles in SLE pathogenesis. This study also highlights the importance of studying DNAm changes in different immune cell lineages rather than only whole blood, since cell type-specific DNAm changes facilitated the elucidation of the cell type-specific molecular pathophysiology of SLE. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1559-2294 1559-2308 15592294 |
| Relation: | https://doaj.org/toc/1559-2294; https://doaj.org/toc/1559-2308 |
| DOI: | 10.1080/15592294.2019.1585176 |
| URL الوصول: | https://doaj.org/article/89d8edee4cdc49f6be155e835dbf1cfd |
| رقم الأكسشن: | edsdoj.89d8edee4cdc49f6be155e835dbf1cfd |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher 
Full Text Finder | تدمد: | 15592294 15592308 |
|---|---|
| DOI: | 10.1080/15592294.2019.1585176 |