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Evaluation of real time PCR for the detection of Mycobacterium avium subsp. paratuberculosis in faecal samples of cattle

التفاصيل البيبلوغرافية
العنوان: Evaluation of real time PCR for the detection of Mycobacterium avium subsp. paratuberculosis in faecal samples of cattle
المؤلفون: R. K. Chaitanya, G. Priyanka, B. Sreedevi
المصدر: Journal of Veterinary and Animal Sciences, Vol 52, Iss 4, Pp 414-417 (2021)
بيانات النشر: Director of Academics and Research, Kerala Veterinary and Animal Sciences University, 2021.
سنة النشر: 2021
المجموعة: LCC:Animal biochemistry
LCC:Science (General)
مصطلحات موضوعية: johne’s disease, map, qpcr, f57, Animal biochemistry, QP501-801, Science (General), Q1-390
الوصف: The efficiency and suitability of a MAP F57 based SYBR Green qPCR assay for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) using a set of known MAP positive (12) and MAP negative (23) DNA samples that were previously identified by conventional IS 900 PCR were assessed. These DNA samples were isolated in our previous study from faecal samples collected from cattle in the livestock farms under government sector with a previous history of Johne’s disease. The MAP F57 qPCR was able to identify all the positive samples accurately and rapidly with Cq values ranging from 20-29.The efficiency of qPCR using recombinant plasmid for standard curve was 0.991 and limit of detection was 10 MAP organisms per microlitre of DNA sample.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 0971-0701
2582-0605
Relation: https://www.jvas.in/abstract/1034; https://doaj.org/toc/0971-0701; https://doaj.org/toc/2582-0605
DOI: 10.51966/jvas.2021.52.4.414-417
URL الوصول: https://doaj.org/article/d8a8c1435b5547cca0059510708b261f
رقم الأكسشن: edsdoj.8a8c1435b5547cca0059510708b261f
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:09710701
25820605
DOI:10.51966/jvas.2021.52.4.414-417