دورية أكاديمية
Biological Activities of Citrus-Derived Extracellular Vesicles on Human Cells: The Role of Preservation
| العنوان: | Biological Activities of Citrus-Derived Extracellular Vesicles on Human Cells: The Role of Preservation |
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| المؤلفون: | Theodora Karamanidou, Konstantinos Krommydas, Maria Karanikou, Dimitrios Tsamos, Konstantinos Michalakis, Dimitris Kletsas, Alexander Tsouknidas, Harris Pratsinis |
| المصدر: | Current Issues in Molecular Biology, Vol 46, Iss 6, Pp 5812-5824 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | tangential flow filtration, fresh EVs, freeze-thawed EVs, freeze-dried EVs, cell proliferation, Akt phosphorylation, Biology (General), QH301-705.5 |
| الوصف: | Extracellular vesicles (EVs) have been identified as important mediators for cell-to-cell communication. Citrus-based EVs in particular offer an excellent platform for nutraceutical delivery systems, as their endemic cargo includes micronutrients (e.g., ascorbic acid), which contribute to their antioxidant capacity. Despite being extensively investigated as to their therapeutic and diagnostic potential, their cargo is inherently unstable and thus directly affected by their storage and preservation. In this study, EVs were isolated from citrus fruit using tangential flow filtration and evaluated for their physicochemical characteristics, antioxidant activity and effects on human cells. To assess how their isolation and preservation methods affect these properties, the EVs were tested immediately after isolation (from fresh and freeze-thawed juices) or following freeze-drying. A measurable biological effect of cryoprotection on citrus-derived EVs was evident, whether during or after isolation. This was more pronounced in the cell-based assays, ranging from −4% to +32% in human skin fibroblast proliferation. Nevertheless, the effects on human cancer cells varied depending on the cell line. Although these results should be considered preliminary observations, subject to further investigation, it is safe to state that any type of preservation is expected to impact the EVs’ biological activity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/46/6/347; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb46060347 |
| URL الوصول: | https://doaj.org/article/8abfe167f15244caa90e287b4abd0f0b |
| رقم الأكسشن: | edsdoj.8abfe167f15244caa90e287b4abd0f0b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14673045 14673037 |
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| DOI: | 10.3390/cimb46060347 |