دورية أكاديمية
أعرض في EDS

Quantifying myelin content in brain tissue using color Spatial Light Interference Microscopy (cSLIM).

التفاصيل البيبلوغرافية
العنوان: Quantifying myelin content in brain tissue using color Spatial Light Interference Microscopy (cSLIM).
المؤلفون: Michael Fanous, Megan P Caputo, Young Jae Lee, Laurie A Rund, Catherine Best-Popescu, Mikhail E Kandel, Rodney W Johnson, Tapas Das, Matthew J Kuchan, Gabriel Popescu
المصدر: PLoS ONE, Vol 15, Iss 11, p e0241084 (2020)
بيانات النشر: Public Library of Science (PLoS), 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Deficient myelination of the brain is associated with neurodevelopmental delays, particularly in high-risk infants, such as those born small in relation to their gestational age (SGA). New methods are needed to further study this condition. Here, we employ Color Spatial Light Interference Microscopy (cSLIM), which uses a brightfield objective and RGB camera to generate pathlength-maps with nanoscale sensitivity in conjunction with a regular brightfield image. Using tissue sections stained with Luxol Fast Blue, the myelin structures were segmented from a brightfield image. Using a binary mask, those portions were quantitatively analyzed in the corresponding phase maps. We first used the CLARITY method to remove tissue lipids and validate the sensitivity of cSLIM to lipid content. We then applied cSLIM to brain histology slices. These specimens are from a previous MRI study, which demonstrated that appropriate for gestational age (AGA) piglets have increased internal capsule myelination (ICM) compared to small for gestational age (SGA) piglets and that a hydrolyzed fat diet improved ICM in both. The identity of samples was blinded until after statistical analyses.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0241084
URL الوصول: https://doaj.org/article/8aef941eb963463aa7236d4e85e82e50
رقم الأكسشن: edsdoj.8aef941eb963463aa7236d4e85e82e50
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0241084