دورية أكاديمية
Age-dependent aggregation of ribosomal RNA-binding proteins links deterioration in chromatin stability with challenges to proteostasis
| العنوان: | Age-dependent aggregation of ribosomal RNA-binding proteins links deterioration in chromatin stability with challenges to proteostasis |
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| المؤلفون: | Julie Paxman, Zhen Zhou, Richard O'Laughlin, Yuting Liu, Yang Li, Wanying Tian, Hetian Su, Yanfei Jiang, Shayna E Holness, Elizabeth Stasiowski, Lev S Tsimring, Lorraine Pillus, Jeff Hasty, Nan Hao |
| المصدر: | eLife, Vol 11 (2022) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | single-cell aging, proteostasis, time-lapse imaging, chromatin stability, microfluidics, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Chromatin instability and protein homeostasis (proteostasis) stress are two well-established hallmarks of aging, which have been considered largely independent of each other. Using microfluidics and single-cell imaging approaches, we observed that, during the replicative aging of Saccharomyces cerevisiae, a challenge to proteostasis occurs specifically in the fraction of cells with decreased stability within the ribosomal DNA (rDNA). A screen of 170 yeast RNA-binding proteins identified ribosomal RNA (rRNA)-binding proteins as the most enriched group that aggregate upon a decrease in rDNA stability induced by inhibition of a conserved lysine deacetylase Sir2. Further, loss of rDNA stability induces age-dependent aggregation of rRNA-binding proteins through aberrant overproduction of rRNAs. These aggregates contribute to age-induced proteostasis decline and limit cellular lifespan. Our findings reveal a mechanism underlying the interconnection between chromatin instability and proteostasis stress and highlight the importance of cell-to-cell variability in aging processes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/75978; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.75978 |
| URL الوصول: | https://doaj.org/article/8b123c5e430f4c03951e121b50a9be75 |
| رقم الأكسشن: | edsdoj.8b123c5e430f4c03951e121b50a9be75 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.75978 |