دورية أكاديمية
An experimental setup to evaluate innovative therapy options for the enhancement of bone healing using BMP as a benchmark – a pilot study
| العنوان: | An experimental setup to evaluate innovative therapy options for the enhancement of bone healing using BMP as a benchmark – a pilot study |
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| المؤلفون: | B Preininger, H Gerigk, J Bruckner, C Perka, H Schell, A Ellinghaus, K Schmidt-Bleek, G Duda |
| المصدر: | European Cells & Materials, Vol 23, Pp 262-272 (2012) |
| بيانات النشر: | AO Research Institute Davos, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Surgery LCC:Diseases of the musculoskeletal system |
| مصطلحات موضوعية: | bone haematoma, bone morphogenetic protein (BMP), delayed healing model, rat, artificial blood clot, Surgery, RD1-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Critical or delayed bone healing in rat osteotomy (OT) models is mostly achieved through large defects or instability. We aimed to design a rat OT model for impaired bone healing based on age, gender and parity. The outcome should be controllable through variations of the haematoma in the OT including a bone morphogenetic protein (BMP) 2 guided positive control.Using external fixation to stabilise femoral a 2 mm double OT in 12 month old, female Sprague Dawley rats after a minimum of 3 litters healing was characterised following in situ haematoma formation (ISH-group)), transplantation of a BMP charged autologous blood clot (BMP-group) and the artificial blood clot only (ABC-group) into the OT-gap. In vivo micro-computer tomography (µCT) scans were performed after 2, 4 and 6 weeks. After 6 weeks specimens underwent histological analyses.In µCT examinations and histological analyses no bony bridging was observed in all but one animal in the ISH-group. In the BMP group complete bridging was achieved in all animals. The ABC-group showed less mineralised tissue formation and smaller bridging scores during the course of healing than the ISH-group.In this pilot study we introduce a model for impaired bone healing taking the major biological risk factors into account. We could show that the in situ fracture haematoma is essential for bone regeneration. Using BMP as a positive control the presented experimental setup can serve to evaluate innovative therapeutical concepts in long bone application. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1473-2262 |
| Relation: | http://www.ecmjournal.org/journal/papers/vol023/pdf/v023a20.pdf; https://doaj.org/toc/1473-2262 |
| URL الوصول: | https://doaj.org/article/8b1db013fbba4b919e50c143d04df7cb |
| رقم الأكسشن: | edsdoj.8b1db013fbba4b919e50c143d04df7cb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14732262 |
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