دورية أكاديمية
Conformational Heterogeneity of Cyclosporin A in Cyclophilin 18 Binding.
| العنوان: | Conformational Heterogeneity of Cyclosporin A in Cyclophilin 18 Binding. |
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| المؤلفون: | Weilin Lin, Andres Quintero, Yixin Zhang |
| المصدر: | PLoS ONE, Vol 11, Iss 4, p e0153669 (2016) |
| بيانات النشر: | Public Library of Science (PLoS), 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The immunosuppressive drug cyclosporin A (CsA) binds to its receptor protein cyclophilin 18 (Cyp18) in two distinct kinetic phases, while the mechanism remains elusive. Stopped-flow measurements coupled with titration and competition experiments were used to investigate the puzzling two-phase process of CsA and Cyp18 interaction. This study leads to the dissection of different conformational fractions of either direct fast binding or slow binding with rate-limiting conformational inter-conversion and the real-time measurement of kon value (8.34 ± 0.22 x106 M-1s-1) in solution. Furthermore, our study indicates that the structure of CsA during dissociation from the protein possesses a distribution of conformations different from those in solution under equilibrium condition. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC4833397?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0153669 |
| URL الوصول: | https://doaj.org/article/d8b2fafa28dd40d298a1031b9cbcfce9 |
| رقم الأكسشن: | edsdoj.8b2fafa28dd40d298a1031b9cbcfce9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0153669 |