دورية أكاديمية

Effect of air–liquid interface on cultured human intestinal epithelial cells

التفاصيل البيبلوغرافية
العنوان: Effect of air–liquid interface on cultured human intestinal epithelial cells
المؤلفون: Akanksha Sabapaty, Po‐Yu Lin, James C. Y. Dunn
المصدر: FASEB BioAdvances, Vol 6, Iss 2, Pp 41-52 (2024)
بيانات النشر: Wiley, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: air‐liquid interface, enteroids, intestinal epithelial cells, monolayer, submerged conditions, Biology (General), QH301-705.5
الوصف: Abstract The intestinal epithelium is a dynamic barrier that allows the selective exchange of ions, hormones, proteins, and nutrients. To accomplish this, the intestinal epithelium adopts a highly columnar morphology which is partially lost in submerged culturing systems. To achieve this, small intestinal tissue samples were utilized to obtain human intestinal crypts to form enteroids. The Transwell system was subsequently employed to form a monolayer of cells that was cultured in either the submerged condition or the air–liquid Interface (ALI) condition. We found that the human intestinal monolayer under the ALI condition exhibited morphology more similar to the normal intestinal epithelium. F‐actin localization and brush border formation were observed apically, and the integrity of the tight junctions was preserved in the ALI condition. Fewer apoptotic cells were observed in the ALI conditions as compared to the submerged conditions. The monolayer of cells expressed a higher level of secretory cell lineage genes in the ALI condition. The ALI condition positively contributes toward a more differentiated phenotype of epithelial cells. It serves as an amplifier that enhances the existing differentiation cue. The ALI system provides a more differentiated platform to study intestinal function compared to submerged conditions.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2573-9832
Relation: https://doaj.org/toc/2573-9832
DOI: 10.1096/fba.2023-00132
URL الوصول: https://doaj.org/article/8b32df81a0144b7eafdc48d9e88092bb
رقم الأكسشن: edsdoj.8b32df81a0144b7eafdc48d9e88092bb
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:25739832
DOI:10.1096/fba.2023-00132