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Effect of Berberine on Bcl-2 Expression in Chronic Lymphocytic Leukemia Compared with Healthy Controls

التفاصيل البيبلوغرافية
العنوان: Effect of Berberine on Bcl-2 Expression in Chronic Lymphocytic Leukemia Compared with Healthy Controls
المؤلفون: Mehrnoosh Pashaei, Mehdi Barati, Farahnaz Ghahremanfad, Fatemeh Pak, Abolghasem Ajami, Parviz Kokhaei
المصدر: Journal of Mazandaran University of Medical Sciences, Vol 28, Iss 170, Pp 56-63 (2019)
بيانات النشر: Mazandaran University of Medical Sciences, 2019.
سنة النشر: 2019
المجموعة: LCC:Medicine
LCC:Medicine (General)
مصطلحات موضوعية: chronic lymphocytic leukemia, bcl-2, berberine, real-time pcr, Medicine, Medicine (General), R5-920
الوصف: Background and purpose: Anti-apoptotic protein Bcl-2 prevents apoptosis through the mitochondrial pathway and high-level expression of Bcl-2 is required for survival of chronic lymphocytic leukemia (CLL). Berberine (BBR) is an alkaloid that induces apoptosis of cancer cells via mitochondrial pathways. The aim of this study was to investigate the effect of Berberine on Bcl-2 gene expression in peripheral blood mononuclear cells (PBMCs) of CLL patients compared to healthy subjects in vitro. Materials and methods: In this experimental study, peripheral blood was obtained from 12 CLL patients and six age-matched healthy subjects. PBMCs were isolated by Ficoll separation and were cultured in two groups for 48 hours incubation; treated with Berberine (25 μM) and untreated. Finally, Bcl-2 gene expression was investigated in both groups following RNA extraction and cDNA synthesis by Real time PCR using SYBR Green method. Results: Bcl-2 gene expression levels in CLL patients treated with Berberine were significantly lower than those of the untreated group (P
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
Persian
تدمد: 1735-9260
1735-9279
Relation: http://jmums.mazums.ac.ir/article-1-12037-en.html; https://doaj.org/toc/1735-9260; https://doaj.org/toc/1735-9279
URL الوصول: https://doaj.org/article/8b3448116112480bb6efcce759545b58
رقم الأكسشن: edsdoj.8b3448116112480bb6efcce759545b58
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17359260
17359279