دورية أكاديمية
Effects of castration on the expression of the NGF and TrkA in the vas deferens and accessory male genital glands of the rat
| العنوان: | Effects of castration on the expression of the NGF and TrkA in the vas deferens and accessory male genital glands of the rat |
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| المؤلفون: | C. Squillacioti, A. De Luca, S. Paino, E. Langella, N. Mirabella |
| المصدر: | European Journal of Histochemistry, Vol 53, Iss 4, Pp e29-e29 (2009) |
| بيانات النشر: | PAGEPress Publications, 2009. |
| سنة النشر: | 2009 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | androgen hormones, stromal cells, immunohistochemistry, real-time RT-PCR, prostate., Biology (General), QH301-705.5 |
| الوصف: | Nerve Growth Factor (NGF) is a member of the neurotrophin family. Neurotrophins exert their effects by binding to corresponding receptors, which are formed by the tyrosine protein kinases TrkA, TrkB, and TrkC, and the low affinity p75NTR receptor. The role of neurotrophins in the biology of male genital organs is far from clear. In particular, little is known about the influence of sex hormones on the expression of neurotrophins and their receptors. In the present study, using immunohistochemistry and real time RT-PCR, we investigated the expression of NGF and TrkA in the vas deferens and accessory male genital glands in normal and castrated rats. In normal rats, both NGF- and TrkA-immunoreactivities (IR) were localized in the epithelial layer of the vas deferens. NGFIR was also found in the stroma and epithelium of the vesicular gland and prostate. TrkA-IR was distributed in the epithelial cells of vesicular and prostate glands. The nerves were weakly immunoreactive in all the examined organs. After castration the immunoreactivities increased. Real-time RT-PCR experiments indicated that NGF and TrkA mRNA levels increased significantly after castration. These results suggest that NGF and TrkA are expressed in the internal male genital organs of the rat and that their expression is downregulated by androgen hormones. We hypothesize NGF and TrkA play a role in the processes that regulate the involution of these organs under conditions of androgen deprivation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1121-760X 2038-8306 |
| Relation: | http://www.ejh.it/index.php/ejh/article/view/1535; https://doaj.org/toc/1121-760X; https://doaj.org/toc/2038-8306 |
| DOI: | 10.4081/ejh.2009.e29 |
| URL الوصول: | https://doaj.org/article/8b583633b8284113a07eeec9b4d0e1a4 |
| رقم الأكسشن: | edsdoj.8b583633b8284113a07eeec9b4d0e1a4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1121760X 20388306 |
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| DOI: | 10.4081/ejh.2009.e29 |