دورية أكاديمية

Sexual Dimorphisms of Protein-Coding Gene Profiles in Placentas From Women With Systemic Lupus Erythematosus

التفاصيل البيبلوغرافية
العنوان: Sexual Dimorphisms of Protein-Coding Gene Profiles in Placentas From Women With Systemic Lupus Erythematosus
المؤلفون: Hui-hui Li, Lin-tao Sai, Shan Tian, Yuan Liu, Colman I. Freel, Kai Wang, Chi Zhou, Jing Zheng, Qiang Shu, Ying-jie Zhao
المصدر: Frontiers in Medicine, Vol 9 (2022)
بيانات النشر: Frontiers Media S.A., 2022.
سنة النشر: 2022
المجموعة: LCC:Medicine (General)
مصطلحات موضوعية: systemic lupus erythematosus, protein-coding RNA, placenta, pregnancy, fetal sex, Medicine (General), R5-920
الوصف: BackgroundSystemic lupus erythematosus (SLE) may cause pathogenic changes in the placentas during human pregnancy, such as decreased placental weight, intraplacental hematoma, ischemic hypoxic change, placental infarction, and decidual vasculopathy, which contribute to high maternal and fetal mortality and morbidity. Sex-specific adaptations of the fetus are associated with SLE pregnancies. The present study aimed to determine the transcriptomic profiles of female and male placentas from women with SLE.MethodsRNA sequencing (RNA-seq) was performed to identify differentially expressed protein-coding genes (DEGs) in placentas from women with SLE vs. normal term (NT) pregnancies with female and male fetuses (n = 3-5/sex/group). Real-time-quantitative PCR was performed (n = 4 /sex/group) to validate the RNA-seq results. Bioinformatics functional analysis was performed to predict the biological functions and pathways of SLE-dysregulated protein-coding genes.ResultsCompared with NT-female (NT-F) placentas, 119 DEGs were identified in SLE-female (SLE-F) placentas. Among these 119 DEGs, five and zero are located on X- and Y-chromosomes, respectively, and four are located on the mitochondrial genome. Compared with NT-male (NT-M) placentas, 458 DEGs were identified in SLE-male (SLE-M) placentas, among which 16 are located on the X-chromosome and zero on the Y-chromosome and mitochondrial genome. Twenty-four DEGs were commonly dysregulated in SLE-F and -M placentas. Functional analysis showed that SLE-dysregulated protein-coding genes were associated with diverse biological functions and pathways, including angiogenesis, cellular response to growth factor stimulus, heparin-binding, HIF (hypoxia-inducible factor)-1 signaling pathway, and Interleukin-17 (IL-17) signaling pathway in both SLE-F and -M placentas. Biological regulations were differentially enriched between SLE-F and -M placentas. Regulation of blood circulation, response to glucocorticoid, and rhythmic process were all enriched in SLE-F, but not SLE-M placentas. In contrast, tumor necrosis factor production, Th17 cell differentiation, and MDA (melanoma differentiation-associated gene)-5 signaling pathway were enriched in SLE-M but not SLE-F placentas.ConclusionThis report investigated the protein-coding gene profiles of placenta tissues from SLE patients using RNA-seq. The results suggest that the SLE-dysregulated protein-coding genes in placentas may contribute to the pathophysiological progress of SLE pregnancies in a fetal sex-specific manner, leading to adverse pregnancy outcomes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2296-858X
Relation: https://www.frontiersin.org/articles/10.3389/fmed.2022.798907/full; https://doaj.org/toc/2296-858X
DOI: 10.3389/fmed.2022.798907
URL الوصول: https://doaj.org/article/e8b91b18e6184363b8e4377c4a4b0874
رقم الأكسشن: edsdoj.8b91b18e6184363b8e4377c4a4b0874
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2296858X
DOI:10.3389/fmed.2022.798907