دورية أكاديمية
High mobilization of CD133+/CD34+ cells expressing HIF-1α and SDF-1α in septic abdominal surgical patients
| العنوان: | High mobilization of CD133+/CD34+ cells expressing HIF-1α and SDF-1α in septic abdominal surgical patients |
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| المؤلفون: | Antonella Cotoia, Olga Cela, Gaetano Palumbo, Sabrina Altamura, Flavia Marchese, Nicoletta Mangialetto, Daniela La Bella, Vincenzo Lizzi, Nazzareno Capitanio, Gilda Cinnella |
| المصدر: | BMC Anesthesiology, Vol 20, Iss 1, Pp 1-8 (2020) |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Anesthesiology |
| مصطلحات موضوعية: | Endothelial progenitor cells, Hypoxia inducible factor-1α, Stromal cell-derived factor-1α, Sepsis, Postoperative abdominal laparoscopic patients, Hematopoietic stem cells, Anesthesiology, RD78.3-87.3 |
| الوصف: | Abstract Background The control of endothelial progenitor cells (CD133+/CD34+ EPCs) migrating from bone marrow to peripheral blood is not completely understood. Emerging evidence suggests that stromal cell-derived factor-1α (SDF-1α) mediates egression of EPCs from bone marrow, while the hypoxia inducible factor (HIF) transcriptional system regulates SDF-1α expression. Our study aimed to investigate the time course of circulating CD133+/CD34+ EPCs and its correlation with the expression of HIF-1α protein and SDF-1α in postoperative laparoscopic abdominal septic patients. Methods Postoperative patients were divided in control (C group) and septic group (S group) operated immediately after the diagnosis of sepsis/septic shock. Blood samples were collected at baseline (0), 1, 3 and 7 postoperative days for CD133+/CD34+ EPCs count expressing or not the HIF-1α and SDF-1α analysis. Results Thirty-two patients in S group and 39 in C group were analyzed. In C group CD133+/CD34+ EPCs count remained stable throughout the study period, increasing on day 7 (173 [0–421] /μl vs baseline: P = 0.04; vs day 1: P = 0.002). In S group CD133+/CD34+ EPCs count levels were higher on day 3 (vs day 1: P = 0.006 and day 7: P = 0.026). HIF-1α expressing CD133+/CD34+ EPCs count decreased on day 1 as compared with the other days in C group (day 0 vs 1: P = 0.003, days 3 and 7 vs 1: P = 0.008), while it was 321 [0–1418] /μl on day 3 (vs day 1; P = 0.004), and 400 [0–587] /μl on day 7 in S group. SDF-1α levels were higher not only on baseline but also on postoperative day 1 in S vs C group (219 [124–337] pg/ml vs 35 [27–325] pg/ml, respectively; P = 0.01). Conclusion Our results indicate that sepsis in abdominal laparoscopic patients might constitute an additional trigger of the EPCs mobilization as compared with non-septic surgical patients. A larger mobilization of CD133+/CD34+ EPCs, preceded by enhanced plasmatic SDF-1α, occurs in septic surgical patients regardless of HIF-1α expression therein. Trial registration ClinicalTrials.gov no. NCT02589535 . Registered 28 October 2015. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2253 98867164 |
| Relation: | http://link.springer.com/article/10.1186/s12871-020-01068-w; https://doaj.org/toc/1471-2253 |
| DOI: | 10.1186/s12871-020-01068-w |
| URL الوصول: | https://doaj.org/article/8c2ac98867164e6b93ec4da406d17cbe |
| رقم الأكسشن: | edsdoj.8c2ac98867164e6b93ec4da406d17cbe |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14712253 98867164 |
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| DOI: | 10.1186/s12871-020-01068-w |