دورية أكاديمية
Electrosprayed highly cross-linked arabinoxylan particles: effect of partly fermentation on the inhibition of Caco-2 cells proliferation
| العنوان: | Electrosprayed highly cross-linked arabinoxylan particles: effect of partly fermentation on the inhibition of Caco-2 cells proliferation |
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| المؤلفون: | Mayra A. Mendez-Encinas, Dora E. Valencia-Rivera, Elizabeth Carvajal-Millan, Humberto Astiazaran-Garcia, Agustín Rascón-Chu, Francisco Brown-Bojorquez |
| المصدر: | AIMS Bioengineering, Vol 8, Iss 1, Pp 52-70 (2021) |
| بيانات النشر: | AIMS Press, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Chemical engineering LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | ferulated arabinoxylan, ferulic acid, antiproliferative activity, Chemical engineering, TP155-156, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Arabinoxylans (AX) are gelling polysaccharides with potential applications as colon-targeted biomaterials. Nevertheless, the fermentation of highly cross-linked AX particles (AXP) by colonic bacteria and the effect of its fermentation supernatants on the proliferation of human colon cancer cells have not been investigated so far. In this study, electrosprayed AXP were fermented by Bifidobacterium longum, Bifidobacterium adolescentis, and Bacteroides ovatus. The effect of AXP fermentation supernatant (AXP-fs) on the inhibition of the human colon cancer cell line Caco-2 proliferation was investigated. AXP presented a mean diameter of 533 µm, a spherical shape, and a cross-linking content (dimers and trimers of ferulic acid) of 1.65 µg/mg polysaccharide. After 48 h of bacteria exposure, AXP were only partly fermented, probably due to polymeric network steric hindrance that limits the access of bacterial enzymes to the polysaccharide target sites. AXP partial fermentation was evidenced by a moderate short-chain fatty acid production (SCFA) (23 mM) and a collapsed and disintegrated microstructure revealed by scanning electron microscopy. AXP-fs exerted slight inhibition of Caco-2 cell proliferation (11%), which could be attributed to the SCFA generated during partly polysaccharide fermentation. These findings indicate that electrosprayed AXP are a slow-fermentable biomaterial presenting slight anti-cancer properties and potential application in colon cancer prevention. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2375-1495 |
| Relation: | https://doaj.org/toc/2375-1495 |
| DOI: | 10.3934/bioeng.2021006?viewType=HTML |
| DOI: | 10.3934/bioeng.2021006 |
| URL الوصول: | https://doaj.org/article/d8c7a10368bb47ef83e745834367b931 |
| رقم الأكسشن: | edsdoj.8c7a10368bb47ef83e745834367b931 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23751495 |
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| DOI: | 10.3934/bioeng.2021006?viewType=HTML |