دورية أكاديمية
Protective effect of recombinant staphylococcal enterotoxin A entrapped in polylactic-co-glycolic acid microspheres against Staphylococcus aureus infection
| العنوان: | Protective effect of recombinant staphylococcal enterotoxin A entrapped in polylactic-co-glycolic acid microspheres against Staphylococcus aureus infection |
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| المؤلفون: | Chen Liben, Li Shuang, Wang Zhengfang, Chang Ruilong, Su Jingliang, Han Bo |
| المصدر: | Veterinary Research, Vol 43, Iss 1, p 20 (2012) |
| بيانات النشر: | BMC, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | Veterinary medicine, SF600-1100 |
| الوصف: | Abstract Staphylococcus aureus is an important cause of nosocomial and community-acquired infections in humans and animals, as well as the cause of mastitis in dairy cattle. Vaccines aimed at preventing S. aureus infection in bovine mastitis have been studied for many years, but have so far been unsuccessful due to the complexity of the bacteria, and the lack of suitable vaccine delivery vehicles. The current study developed an Escherichia coli protein expression system that produced a recombinant staphylococcal enterotoxin A (rSEA) encapsulated into biodegradable microparticles generated by polylactic-co-glycolic acid (PLGA) dissolved in methylene chloride and stabilized with polyvinyl acetate. Antigen loading and surface properties of the microparticles were investigated to optimize particle preparation protocols. The prepared PLGA-rSEA microspheres had a diameter of approximately 5 μm with a smooth and regular surface. The immunogenicity of the PLGA-rSEA vaccine was assessed using mice as an animal model and showed that the vaccine induced a strong humoral immune response and increased the percent survival of challenged mice and bacterial clearance. Histological analysis showed moderate impairment caused by the pathogen upon challenge afforded by immunization with PLGA-rSEA microspheres. Antibody titer in the sera of mice immunized with PLGA-rSEA microparticles was higher than in vaccinated mice with rSEA. In conclusion, the PLGA-rSEA microparticle vaccine developed here could potentially be used as a vaccine against enterotoxigenic S. aureus. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1297-9716 0928-4249 |
| Relation: | http://www.veterinaryresearch.org/content/43/1/20; https://doaj.org/toc/0928-4249; https://doaj.org/toc/1297-9716 |
| DOI: | 10.1186/1297-9716-43-20 |
| URL الوصول: | https://doaj.org/article/8cca8405b348430b8c7226e3b54aea36 |
| رقم الأكسشن: | edsdoj.8cca8405b348430b8c7226e3b54aea36 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 12979716 09284249 |
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| DOI: | 10.1186/1297-9716-43-20 |