دورية أكاديمية
Biochemical characterization of trypsin from Indonesian skipjack tuna (Katsuwonus pelamis) viscera
| العنوان: | Biochemical characterization of trypsin from Indonesian skipjack tuna (Katsuwonus pelamis) viscera |
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| المؤلفون: | Dali Faiza A., Nurjanah Nurjanah, Lioe Hanifah N., Suhartono Maggy T. |
| المصدر: | Open Agriculture, Vol 9, Iss 1, Pp 542-52 (2024) |
| بيانات النشر: | De Gruyter, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Agriculture LCC:Agriculture (General) |
| مصطلحات موضوعية: | biochemical characteristics, fish viscera, fractionation, trypsin, Agriculture, Agriculture (General), S1-972 |
| الوصف: | Trypsin production from skipjack tuna (Katsuwonus pelamis) viscera is one significant way to increase the value of fish’s industrial waste. The present work reports the biochemical properties of trypsin from skipjack tuna viscera. The trypsin was fractionated using 0–60% ammonium sulfate and dialyzed. The enzyme was characterized to find the optimum temperature and pH for the substrate N-α-benzoyl-dl-arginine-p-nitroanilide. The 40–50% ammonium sulfate fractionation showed the highest activity at a specific activity of 1.66 U/mg and yield of 69.91%. Specific activity increased after dialysis to 2.17 U/mg with 4.49 times purity and yield of 39.20%. The molecular weights of the enzymes were estimated as 25, 29, and 35 kDa based on the enzyme activity separated by electrophoresis. The enzyme worked optimally at a temperature and pH of 50–60°C and 8.0, respectively. Metal ions (Ca2+, K+, Na+, Mg2+) at a concentration of 20 mM showed no influence on the activity. Enzyme activity was inhibited by Zn2+ at 20 mM, phenyl methyl sulfonyl fluoride (PMSF), benzamidine, and soybean trypsin inhibitor (SBTI), which confirmed the characteristics of a serine protease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2391-9531 2022-0308 |
| Relation: | https://doaj.org/toc/2391-9531 |
| DOI: | 10.1515/opag-2022-0308 |
| URL الوصول: | https://doaj.org/article/c8dab436f64f4d10ac2adcf62c9f1622 |
| رقم الأكسشن: | edsdoj.8dab436f64f4d10ac2adcf62c9f1622 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23919531 20220308 |
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| DOI: | 10.1515/opag-2022-0308 |