دورية أكاديمية
Bioactive peptides in the pancreatin-hydrolysates of whey protein support cell proliferation and scavenge reactive oxygen species
| العنوان: | Bioactive peptides in the pancreatin-hydrolysates of whey protein support cell proliferation and scavenge reactive oxygen species |
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| المؤلفون: | Haesoo Jung, Damin Jung, Jaehoon Lee, Woojin Ki, Jung-Min Lee, Eun-Mi Kim, Myoung Soo Nam, Kee K. Kim |
| المصدر: | Animal Cells and Systems, Vol 26, Iss 5, Pp 232-242 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | milk, whey protein, hydrolysis, inflammation, cellular senescence, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Whey protein (WP) in milk shows physiologically active functions such as cholesterol control and immune system strengthening. In this study, we performed hydrolysis and peptide polarity fractionation to enhance the efficacy and diversity of its physiological activities, using the digesting enzyme, pancreatin. Our results indicate that hydrolysis significantly increased the cell proliferation of the WP fractions, with the lower-polarity fractions showing greater efficacy in this regard. Our results indicate that hydrolysis significantly increases cell proliferation of the WP fractions. Additionally, we confirmed differences in the antioxidant activity of the WP fractions as a function of polarity was confirmed via scavenging 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay in vitro. WP itself did not show anti-inflammatory efficacy. However, all the hydrolyzed fractions downregulated the mRNA expression levels of inflammatory cytokines in all treated cell lines and, based on a senescence-associated (SA)-β-galactosidase assay, the fraction with the lowest polarity (F6) inhibited cellular senescence to the greatest extent. Furthermore, we identified the peptide sequences with various physiological activities from whey protein hydrolysates through mass spectrometry. Taken together, our results indicate that the fractionation of WP via hydrolysis generates novel functions including promoting cellular cell proliferation, anti-inflammatory effects, and enhancing antioxidant and anti-cellular senescence. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 19768354 2151-2485 1976-8354 |
| Relation: | https://doaj.org/toc/1976-8354; https://doaj.org/toc/2151-2485 |
| DOI: | 10.1080/19768354.2022.2130425 |
| URL الوصول: | https://doaj.org/article/c8e1997661324728b2601fc1eced8e4d |
| رقم الأكسشن: | edsdoj.8e1997661324728b2601fc1eced8e4d |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 19768354 21512485 |
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| DOI: | 10.1080/19768354.2022.2130425 |