دورية أكاديمية
Evaluation of The Relationship among The Levels of SIRT1 and SIRT3 with Oxidative Stress and DNA Fragmentation in Asthenoteratozoospermic Men
| العنوان: | Evaluation of The Relationship among The Levels of SIRT1 and SIRT3 with Oxidative Stress and DNA Fragmentation in Asthenoteratozoospermic Men |
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| المؤلفون: | Abolfazl Nasiri, Asad Vaisi-Raygani, Zohreh Rahimi, Mitra Bakhtiari, Fariborz Bahrehmand, Amir Kiani, Hadi Mozafari, Tayebeh Pourmotabbed |
| المصدر: | International Journal of Fertility and Sterility, Vol 15, Iss 2, Pp 135-140 (2021) |
| بيانات النشر: | Royan Institute (ACECR), Tehran, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | dna fragmentation, infertility, oxidative stress, sirt1, sirt3, Medicine (General), R5-920 |
| الوصف: | Background: Reactive oxygen species (ROS) play a crucial role in etiology of DNA fragmentation and lipid peroxidationin sperm, leading to infertility in men. The silent information regulators SIRT1 and SIRT3 are members of thesirtuins protein family known to be involved in cancer genetics, aging and oxidative stress responses. The aim ofthisstudy is to determine the correlation between SIRT1 and SIRT3 with antioxidants, oxidative stress biomarkers, andDNA fragmentation in the semen of asthenoteratozoospermic and normozoospermic men.Materials and Methods: In this case-control study, after spermogram analysis the specimens were divided intotwo groups, normozospermic (n=40) and asthenoteratozoospermic (n=40), according to World Health Organization(WHO) standards. Sperm DNA fragmentation was evaluatedusing the sperm chromatin dispersion (SCD) test.Catalaseactivity was measured using the Aebi spectrophotometeric method. Total antioxidant capacity (TAC) level and superoxidedismutase (SOD) activitywere measured by using commercially available colorimetric assays. Enzyme-linkedimmune sorbent assay (ELISA) was used to measure SIRT1 and SIRT3 protein levels of seminal plasma. Malondialdehyde(MDA) level in seminal plasma was determined by high-performance liquid chromatography (HPLC).Results: The asthenoteratozoospermic group had significantly lower catalase and SOD activities and TAC levels incomparison with the normozoospermic group (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2008-076X 2008-0778 |
| Relation: | https://www.ijfs.ir/article_46366_bead94d3c252105793ef9293c1707728.pdf; https://doaj.org/toc/2008-076X; https://doaj.org/toc/2008-0778 |
| DOI: | 10.22074/ijfs.2020.134692 |
| URL الوصول: | https://doaj.org/article/ca8e56afed574b04ad79d3d1c5b62867 |
| رقم الأكسشن: | edsdoj.8e56afed574b04ad79d3d1c5b62867 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2008076X 20080778 |
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| DOI: | 10.22074/ijfs.2020.134692 |