دورية أكاديمية
Transcriptional analysis of gasoline engine exhaust particulate matter 2.5-exposed human umbilical vein endothelial cells reveals the different gene expression patterns related to the cardiovascular diseases
| العنوان: | Transcriptional analysis of gasoline engine exhaust particulate matter 2.5-exposed human umbilical vein endothelial cells reveals the different gene expression patterns related to the cardiovascular diseases |
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| المؤلفون: | Inkyo Jung, Minhan Park, Myong-Ho Jeong, Kihong Park, Won-Ho Kim, Geun-Young Kim |
| المصدر: | Biochemistry and Biophysics Reports, Vol 29, Iss , Pp 101190- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Gasoline engine exhaust particulate matter 2.5, HUVECs, RNA sequencing, Transcriptomics, Cardiovascular diseases, Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| الوصف: | Particulate matter (PM) causes several diseases, including cardiovascular diseases (CVDs). Previous studies compared the gene expression patterns in airway epithelial cells and keratinocytes exposed to PM. However, analysis of differentially expressed gene (DEGs) in endothelial cells exposed to PM2.5 (diameter less than 2.5 μm) from fossil fuel combustion has been limited. Here, we exposed human umbilical vein endothelial cells (HUVECs) to PM2.5 from combustion of gasoline, performed RNA-seq analysis, and identified DEGs. Exposure to the IC50 concentrations of gasoline engine exhaust PM2.5 (GPM) for 24 h yielded 1081 (up-regulation: 446, down-regulation: 635) DEGs. The most highly up-regulated gene is NGFR followed by ADM2 and NUPR1. The most highly down-regulated gene is TNFSF10 followed by GDF3 and EDN1. Gene Ontology enrichment analysis revealed that GPM regulated genes involved in cardiovascular system development, tube development and circulatory system development. Kyoto Encyclopedia of Genes and Genomes and Reactome pathway analyses showed that genes related to cytokine–cytokine receptor interactions and cytokine signaling in the immune system were significantly affected by GPM. We confirmed the RNA-seq data of some highly altered genes by qRT-PCR and showed the induction of NGFR, ADM2 and IL-11 at a protein level, indicating that the observed gene expression patterns were reliable. Given the adverse effects of PM2.5 on CVDs, our findings provide new insight into the importance of several DEGs and pathways in GPM-induced CVDs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-5808 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580821002843; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2021.101190 |
| URL الوصول: | https://doaj.org/article/8e6d5c6dde344fce8433e8b78c647ab5 |
| رقم الأكسشن: | edsdoj.8e6d5c6dde344fce8433e8b78c647ab5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24055808 |
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| DOI: | 10.1016/j.bbrep.2021.101190 |