دورية أكاديمية
أعرض في EDS

Non-invasive Diagnostic of Helicobacter pylori in Stools by Nested-qPCR

التفاصيل البيبلوغرافية
العنوان: Non-invasive Diagnostic of Helicobacter pylori in Stools by Nested-qPCR
المؤلفون: María I. Taborda, Gisela Aquea, Yenny Nilo, Karla Salvatierra, Nicolás López, Sergio López, Gustavo Bresky, Juan A. Madariaga, Vittorio Zaffiri, Sergio Häberle, Giuliano Bernal
المصدر: Polish Journal of Microbiology, Vol 67, Iss 1 (2018)
بيانات النشر: Sciendo, 2018.
سنة النشر: 2018
المجموعة: LCC:Genetics
LCC:Microbiology
مصطلحات موضوعية: Helicobacter pylori, molecular diagnostics, nested-qPCR, stools, Genetics, QH426-470, Microbiology, QR1-502
الوصف: The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Rapid Urease Testing (RUT) and histology by Giemsa stain. A fecal sample for nested-qPCR analysis was also obtained. DNA was extracted from the fecal samples, and conventional PCR followed by qPCR of the ureC gene of H. pylori was carried out. We evaluated the presence of H. pylori, in 103 females and 40 males, mean (± SD) age of 56.5 ± 14.18. The sensitivity of RUT to detect the infection was 67.0% (95% C.I.: 57.2 – 75.8) and specificity was 92.3% (95% C.I.: 76.5 – 99.1). Histology by Giemsa stain, commonly used as a reference for H. pylori detection, showed a sensitivity of 98.6% (95% C.I.: 92.5 – 100.0) and a specificity of 89.7% (95% C.I.: 72.7 – 97.8). In contrast, detection of H. pylori infection in stools by nested-qPCR showed a sensitivity of 100% (95% C.I.: 94.9 – 100.0) and a specificity of 83.9% (95% C.I.: 66.3 – 94.6). Our test, based in nested-qPCR is a better diagnostic alternative than conventional RUT, and is similar to histology by Giemsa stain in the detection of H. pylori, by which the test could be used for non-invasive diagnosis in clinical practice.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1733-1331
2544-4646
Relation: https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/67/1/pdf/10.5604_01.3001.0011.5881.pdf; https://doaj.org/toc/1733-1331; https://doaj.org/toc/2544-4646
DOI: 10.5604/01.3001.0011.5881
URL الوصول: https://doaj.org/article/8ee9d4232e8b4e13b057582e6e10fe33
رقم الأكسشن: edsdoj.8ee9d4232e8b4e13b057582e6e10fe33
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17331331
25444646
DOI:10.5604/01.3001.0011.5881