دورية أكاديمية
Synthetic Diversity and Catalytic Mechanism of Peptide Dendrimers
| العنوان: | Synthetic Diversity and Catalytic Mechanism of Peptide Dendrimers |
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| المؤلفون: | Estelle Delort, Tamis Darbre, Jean-Louis Reymond |
| المصدر: | CHIMIA, Vol 59, Iss 3 (2005) |
| بيانات النشر: | Swiss Chemical Society, 2005. |
| سنة النشر: | 2005 |
| المجموعة: | LCC:Chemistry |
| مصطلحات موضوعية: | Dendrimer, Enzyme model, Ester hydrolysis, Peptide, Solid-phase synthesis, Chemistry, QD1-999 |
| الوصف: | Peptide dendrimers composed of alternating sequences of natural amino acids and branching diamino acids are investigated as synthetic enzyme models. The dendrimers can be prepared by solid-phase peptide synthesis and are obtained pure in yields of 5–35%. Peptide dendrimers with surface histidine residues catalyze ester hydrolysis reaction with enzyme-like kinetics, including substrate binding (KM), catalytic turnover (kcat), and rate acceleration kcat/kuncat = 1000–20'000. Mechanistic investigation by substrate variation, pH-profile, and isothermaltitration calorimetry show that the catalytic effect is caused by positive interaction between the histidine side-chains and creation of a hydrophobic microenvironment for substrate binding. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | German English French |
| تدمد: | 0009-4293 2673-2424 |
| Relation: | https://chimia.ch/chimia/article/view/3950; https://doaj.org/toc/0009-4293; https://doaj.org/toc/2673-2424 |
| DOI: | 10.2533/000942905777676768 |
| URL الوصول: | https://doaj.org/article/8f8da34cfd32412c9c27b0a298961b4f |
| رقم الأكسشن: | edsdoj.8f8da34cfd32412c9c27b0a298961b4f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 00094293 26732424 |
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| DOI: | 10.2533/000942905777676768 |