دورية أكاديمية
Local tissue reaction and histopathological characteristics of three different bulking agents: a rabbit model
| العنوان: | Local tissue reaction and histopathological characteristics of three different bulking agents: a rabbit model |
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| المؤلفون: | Shabnam Sabetkish, Mohammad Javad Mohseni, Nastaran Sabetkish, Abdol-Mohammad Kajbafzadeh |
| المصدر: | International Brazilian Journal of Urology, Vol 47, Iss 2, Pp 322-332 (2021) |
| بيانات النشر: | Sociedade Brasileira de Urologia, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | dextranomer [Supplementary Concept], Bulkamid [Supplementary Concept], Acellular Dermis, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | ABSTRACT Purpose: We assessed the efficacy and safety of a single injection of three bulking agents over the short- and long-term follow-ups in rabbits. Dermal and preputial matrices were compared with Deflux (DxHA) injection. Material and methods: Twenty-four rabbits were divided into three groups. Group I (n=8) underwent the injection of a lyophilized dermal matrix (LDM) beneath the seromuscular layer of the bladder wall. Rabbits in group II (n=8) were injected with lyophilized preputial matrix (LPM). Rabbits of group III (n=8) were injected with DxHA as the control group. They were followed up for 1 and 6 months after the injection. Subcutaneous injection of all bulking agents was also performed in nude mice. Biopsies were stained with LCA (leukocyte common antibody), CD68, CD31, and CD34. Scanning electron microscopy (SEM) and MTT assay were also performed. Results: Immunohistochemistry staining with CD68 and LCA revealed higher inflammation grade in LDM as compared with LPM and DxHA. Fibrosis grade was also higher in LDM both in short- and long-term follow-ups. However, no significant difference was detected in CD31 and CD34 staining between control and experimental groups. SEM analysis showed that the particle size of LPM was more similar to DxHA. MTT assay revealed that cell proliferation was similar in DxHA, LDM, and LPM. In-vivo assay in nude mice model showed more promising results in LPM as compared with LDM. Conclusion: The long-term results demonstrated that LPM was more similar to Deflux with the least local tissue reaction, inflammation, and fibrosis grade. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1677-6119 1677-5538 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382021000200322&tlng=en; http://www.scielo.br/pdf/ibju/v47n2/1677-6119-ibju-47-02-0322.pdf; https://doaj.org/toc/1677-6119 |
| DOI: | 10.1590/s1677-5538.ibju.2020.0171 |
| URL الوصول: | https://doaj.org/article/8f92d47663d94f88a02e213cedcefd01 |
| رقم الأكسشن: | edsdoj.8f92d47663d94f88a02e213cedcefd01 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16776119 16775538 |
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| DOI: | 10.1590/s1677-5538.ibju.2020.0171 |