دورية أكاديمية
Imaging conformations of holo- and apo-transferrin on the single-molecule level by low-energy electron holography
| العنوان: | Imaging conformations of holo- and apo-transferrin on the single-molecule level by low-energy electron holography |
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| المؤلفون: | Hannah Ochner, Sven Szilagyi, Moritz Edte, Tim K. Esser, Stephan Rauschenbach, Luigi Malavolti, Klaus Kern |
| المصدر: | Scientific Reports, Vol 13, Iss 1, Pp 1-8 (2023) |
| بيانات النشر: | Nature Portfolio, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Conformational changes play a key role in the biological function of many proteins, thereby sustaining a multitude of processes essential to life. Thus, the imaging of the conformational space of proteins exhibiting such conformational changes is of great interest. Low-energy electron holography (LEEH) in combination with native electrospray ion beam deposition (ES-IBD) has recently been demonstrated to be capable of exploring the conformational space of conformationally highly variable proteins on the single-molecule level. While the previously studied conformations were induced by changes in environment, it is of relevance to assess the performance of this imaging method when applied to protein conformations inherently tied to a function-related conformational change. We show that LEEH imaging can distinguish different conformations of transferrin, the major iron transport protein in many organisms, by resolving a nanometer-scale cleft in the structure of the iron-free molecule (apo-transferrin) resulting from the conformational change associated with the iron binding/release process. This, along with a statistical analysis of the data, which evidences a degree of flexibility of the molecules, indicates that LEEH is a viable technique for imaging function-related conformational changes in individual proteins. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-023-37116-x |
| URL الوصول: | https://doaj.org/article/8ff1be818e9641b7b1fefe41039a5949 |
| رقم الأكسشن: | edsdoj.8ff1be818e9641b7b1fefe41039a5949 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-023-37116-x |