Summary: Learning, memorizing, and recalling of potential ovipositing sites can influence oviposition preference. Classical conditioning experiments have shown that vinegar flies can learn the association of olfactory, gustatory, or visual stimuli with either positive or negative unconditioned stimuli. However, less is known about whether similar associations are formed in an ecologically more relevant context like during oviposition. Our experiments reveal that Drosophila melanogaster females increase their preference for substrates they have already experienced. However, this change of preference requires that the flies not only smelled or touched the substrates but also oviposited on them. We furthermore show that such an experience results in long-term memory lasting for at least 4 days, i.e., a duration that so far was shown only for aversive conditioning. Our study thus reveals a different form of associative learning in D. melanogaster that might be highly relevant for settling novel ecological niches.
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