دورية أكاديمية

Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq

التفاصيل البيبلوغرافية
العنوان: Surface Ultrastructure and Molecular Studies of Clinostomum complanatum (Rudolphi, 1814) Braun, 1899 (Trematoda: Clinostomidae) Metacercariae in some Freshwater Fishes from Sulaimani Province, Iraq
المؤلفون: Younis S. Abdullah, Samir J. Bilal, Taeeb A. Hama Soor, Yousif O. Mohammad
المصدر: Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ, Vol 36, Iss 2 (2023)
بيانات النشر: University of Basrah, 2023.
سنة النشر: 2023
المجموعة: LCC:Agriculture (General)
مصطلحات موضوعية: Digenea, Freshwater fishes, Genetics, Iraq, Metacercaria, Yellow grub disease, Agriculture (General), S1-972
الوصف: Clinostomum is a trematode genus in the family Clinostomidae. The mentioned trematode species is parasitizing many fish species as intermediate hosts, while piscivorous birds and mammals are the main definitive hosts. Total of 58 metacercariae of Clinostomum larvae were dissected out from 25 infected fish specimens from eight different species from a number of water bodies of Sulaimani Province, Kurdistan Region, Iraq. In this investigation, 959 fish specimens were collected. These includes five cyprinid species: Capoeta trutta (Heckel), C. umbla (Heckel), Carasobarbus kosswigi (Ladiges), Cyprinion macrostomum Heckel and Garra rufa (Heckel), two leuciscid species: Alburnus sellal Heckel and Squalius lepidus Heckel and the mugilid fish Planiliza abu (Heckel). The prevalence of infection for each of these species was 1.8%, 5%, 20%, 1.5%, 1.7%, 4.9%, 4.9% and 1.3%, respectively. The morphology of the Clinostomum metacercariae was studied by using a compound microscope; their ultra-morphology was evaluated with a scanning electron microscopy (SEM); and the molecular analysis were performed by amplifying, sequencing and comparing the ITS1-5.8S-ITS2 and 28S rDNA gene loci from isolated Clinostomum metacercariae. The obtained sequences confirmed that all metacercariae of the Clinostomum, collected during the present study, represented with C. complanatum (Rudolphi, 1814) based on percent identity with sequences in the GenBank subject database. The molecular characterisations of the C. complanatum metacercariae in the present study were deposited in GenBank NCBI.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1814-5868
2520-0860
Relation: https://www.bjas.bajas.edu.iq/index.php/bjas/article/view/1155; https://doaj.org/toc/1814-5868; https://doaj.org/toc/2520-0860
URL الوصول: https://doaj.org/article/90bdb7256e264a49b41bbfc96f50b320
رقم الأكسشن: edsdoj.90bdb7256e264a49b41bbfc96f50b320
قاعدة البيانات: Directory of Open Access Journals