دورية أكاديمية
A novel paper MAP method for rapid high resolution histological analysis
| العنوان: | A novel paper MAP method for rapid high resolution histological analysis |
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| المؤلفون: | Mirae Lee, Jiwon Woo, Doh-Hee Kim, Yu-Mi Yang, Eunice Yoojin Lee, Jung-Hee Kim, Seok-Gu Kang, Jin-Kyung Shim, Jeong-Yoon Park |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-021-02632-1 |
| URL الوصول: | https://doaj.org/article/90d7f1a0de4d40a28c4ee4a10f9ba55a |
| رقم الأكسشن: | edsdoj.90d7f1a0de4d40a28c4ee4a10f9ba55a |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-021-02632-1 |