دورية أكاديمية
A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose
العنوان: | A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose |
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المؤلفون: | Ravneet Mandair, Pinar Karagoz, Roslyn M. Bill |
المصدر: | Applied Sciences, Vol 11, Iss 11, p 4877 (2021) |
بيانات النشر: | MDPI AG, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Technology LCC:Engineering (General). Civil engineering (General) LCC:Biology (General) LCC:Physics LCC:Chemistry |
مصطلحات موضوعية: | enzymatic cascade reaction, malate production, malate dehydrogenase, glucose dehydrogenase, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999 |
الوصف: | A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase. By combining mutant malate dehydrogenase with glucose dehydrogenase from Sulfolobus solfataricus and NAD+/NADH in a closed reaction environment, gluconate and malate could be produced from pyruvate and glucose. After 3 h, the yield of malate was 15.96 mM. These data demonstrate the feasibility of a closed system capable of cofactor regeneration in the production of platform chemicals. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2076-3417 |
Relation: | https://www.mdpi.com/2076-3417/11/11/4877; https://doaj.org/toc/2076-3417 |
DOI: | 10.3390/app11114877 |
URL الوصول: | https://doaj.org/article/912a3d2a0f894695b2165dfc35924485 |
رقم الأكسشن: | edsdoj.912a3d2a0f894695b2165dfc35924485 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20763417 |
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DOI: | 10.3390/app11114877 |