دورية أكاديمية
Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation
| العنوان: | Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation |
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| المؤلفون: | Marta Carroni, Eva Kummer, Yuki Oguchi, Petra Wendler, Daniel K Clare, Irmgard Sinning, Jürgen Kopp, Axel Mogk, Bernd Bukau, Helen R Saibil |
| المصدر: | eLife, Vol 3 (2014) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | single particle EM, ClpB/Hsp104, protein unfolding, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The hexameric AAA+ chaperone ClpB reactivates aggregated proteins in cooperation with the Hsp70 system. Essential for disaggregation, the ClpB middle domain (MD) is a coiled-coil propeller that binds Hsp70. Although the ClpB subunit structure is known, positioning of the MD in the hexamer and its mechanism of action are unclear. We obtained electron microscopy (EM) structures of the BAP variant of ClpB that binds the protease ClpP, clearly revealing MD density on the surface of the ClpB ring. Mutant analysis and asymmetric reconstructions show that MDs adopt diverse positions in a single ClpB hexamer. Adjacent, horizontally oriented MDs form head-to-tail contacts and repress ClpB activity by preventing Hsp70 interaction. Tilting of the MD breaks this contact, allowing Hsp70 binding, and releasing the contact in adjacent subunits. Our data suggest a wavelike activation of ClpB subunits around the ring. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/02481; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.02481 |
| URL الوصول: | https://doaj.org/article/a9145d0f6eb74b92badedf42e5a4d329 |
| رقم الأكسشن: | edsdoj.9145d0f6eb74b92badedf42e5a4d329 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.02481 |