دورية أكاديمية
Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay.
العنوان: | Impact of selective immune-cell depletion on growth of Mycobacterium tuberculosis (Mtb) in a whole-blood bactericidal activity (WBA) assay. |
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المؤلفون: | Gail B Cross, Benjamin C-M Yeo, Paul Edward Hutchinson, Mark C Tan, Rupangi Verma, Qingshu Lu, Nicholas I Paton |
المصدر: | PLoS ONE, Vol 14, Iss 5, p e0216616 (2019) |
بيانات النشر: | Public Library of Science (PLoS), 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Medicine LCC:Science |
مصطلحات موضوعية: | Medicine, Science |
الوصف: | We investigated the contribution of host immune cells to bacterial killing in a whole-blood bactericidal activity (WBA) assay, an ex vivo model used to test efficacy of drugs against mycobacterium tuberculosis (Mtb). We performed WBA assays with immuno-magnetic depletion of specific cell types, in the presence or absence of rifampicin. Innate immune cells decreased Mtb growth in absence of drug, but appeared to diminish the cidal activity of rifampicin, possibly attributable to intracellular bacterial sequestration. Adaptive immune cells had no effect with or without drug. The WBA assay may have potential for testing adjunctive host-directed therapies acting on phagocytic cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1932-6203 |
Relation: | https://doaj.org/toc/1932-6203 |
DOI: | 10.1371/journal.pone.0216616 |
URL الوصول: | https://doaj.org/article/9159a12acb5448a281ddcfbf7805ab7e |
رقم الأكسشن: | edsdoj.9159a12acb5448a281ddcfbf7805ab7e |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 19326203 |
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DOI: | 10.1371/journal.pone.0216616 |