دورية أكاديمية
Optimization of Saccharomyces boulardii production in solid-state fermentation with response surface methodology
| العنوان: | Optimization of Saccharomyces boulardii production in solid-state fermentation with response surface methodology |
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| المؤلفون: | Yuanliang Hu, Huanhuan Qin, Zhichun Zhan, Yaohao Dun, Ying Zhou, Nan Peng, Huayun Ling, Yunxiang Liang, Shumiao Zhao |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 30, Iss 1, Pp 173-179 (2016) |
| بيانات النشر: | Taylor & Francis Group, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | Box–Behnken design, Saccharomyces boulardii, solid-state fermentation, response surface method, yeast count, Biotechnology, TP248.13-248.65 |
| الوصف: | Saccharomyces boulardii preparations are promising probiotics and clinical agents for animals and humans. This work focused on optimizing the nutritional conditions for the production of S. boulardii in solid-state fermentation by using classical and statistical methods. In single-factor experiments, the S. boulardii production was significantly increased by the addition of glucoamylase and the optimal carbon and nitrogen sources were found to be soluble starch and NH4Cl, respectively. The effects of the glucoamylase, soluble starch and NH4Cl on S. boulardii production were evaluated by a three-level three-factor Box–Behnken design and response surface methodology (RSM). The maximal yeast count (4.50 ×109CFU/g) was obtained under the optimized conditions (198 U/g glucoamylase, 2.37% soluble starch and 0.9% NH4Cl), which was in a good agreement with the predicted value of the model. This study has provided useful information on how to improve the accumulation of yeast cells by RSM. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1310-2818 1314-3530 13102818 |
| Relation: | https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530 |
| DOI: | 10.1080/13102818.2015.1086689 |
| URL الوصول: | https://doaj.org/article/91630c8a35d744cf889f1c96e4fad57c |
| رقم الأكسشن: | edsdoj.91630c8a35d744cf889f1c96e4fad57c |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 13102818 13143530 |
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| DOI: | 10.1080/13102818.2015.1086689 |