دورية أكاديمية
أعرض في EDS

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

التفاصيل البيبلوغرافية
العنوان: Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light
المؤلفون: Marek Feith, Tomáš Vičar, Jaromír Gumulec, Martina Raudenská, Anette Gjörloff Wingren, Michal Masařík, Jan Balvan
المصدر: Applied Sciences, Vol 10, Iss 7, p 2597 (2020)
بيانات النشر: MDPI AG, 2020.
سنة النشر: 2020
المجموعة: LCC:Technology
LCC:Engineering (General). Civil engineering (General)
LCC:Biology (General)
LCC:Physics
LCC:Chemistry
مصطلحات موضوعية: holographic microscopy, quantitative phase imaging, blue light, cell mass, cell motility, cell death, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
الوصف: Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm2, 208 mJ/cm2 and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm2 × 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm2 × 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-3417
Relation: https://www.mdpi.com/2076-3417/10/7/2597; https://doaj.org/toc/2076-3417
DOI: 10.3390/app10072597
URL الوصول: https://doaj.org/article/91bfa8fc511b4234a7912d725de3b91b
رقم الأكسشن: edsdoj.91bfa8fc511b4234a7912d725de3b91b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20763417
DOI:10.3390/app10072597