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Novel epitopes identified from efflux pumps of Mycobacterium tuberculosis could induce cytotoxic T lymphocyte response

التفاصيل البيبلوغرافية
العنوان: Novel epitopes identified from efflux pumps of Mycobacterium tuberculosis could induce cytotoxic T lymphocyte response
المؤلفون: Ming-xia Zhai, Fei Chen, Yuan-yuan Zhao, Ya-hong Wu, Guo-dong Li, Yan-feng Gao, Yuan-ming Qi
المصدر: PeerJ, Vol 3, p e1229 (2015)
بيانات النشر: PeerJ Inc., 2015.
سنة النشر: 2015
المجموعة: LCC:Medicine
LCC:Biology (General)
مصطلحات موضوعية: Cytotoxic T lymphocytes, Efflux pump, Epitope, Immunotherapy, Mycobacterium tuberculosis, Medicine, Biology (General), QH301-705.5
الوصف: Overcoming drug-resistance is one of the major challenges to control tuberculosis (TB). The up-regulation of efflux pumps is one common mechanism that leads to drug-resistance. Therefore, immunotherapy targeting these efflux pump antigens could be promising strategy to be combined with current chemotherapy. Considering that CD8+ cytotoxic T lymphocytes (CTLs) induced by antigenic peptides (epitopes) could elicit HLA-restricted anti-TB immune response, efflux pumps from classical ABC family (Mycobacterium tuberculosis, Mtb) were chosen as target antigens to identify CTL epitopes. HLA-A2 restricted candidate peptides from Rv2937, Rv2686c and Rv2687c of Mycobacterium tuberculosis were predicted, synthesized and tested. Five peptides could induce IFN-γ release and cytotoxic activity in PBMCs from HLA-A2+ PPD+ donors. Results from HLA-A2/Kb transgenic mice immunization assay suggested that four peptides Rv2937-p168, Rv2937-p266, Rv2686c-p151, and Rv2686c-p181 could induce significant CTL response in vivo. These results suggested that these novel epitopes could be used as immunotherapy candidates to TB drug-resistance.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2167-8359
Relation: https://peerj.com/articles/1229.pdf; https://peerj.com/articles/1229/; https://doaj.org/toc/2167-8359
DOI: 10.7717/peerj.1229
URL الوصول: https://doaj.org/article/91e9c5149e3144fbbab41d8d6ed6864c
رقم الأكسشن: edsdoj.91e9c5149e3144fbbab41d8d6ed6864c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21678359
DOI:10.7717/peerj.1229