دورية أكاديمية
Population genetic analysis of the Plasmodium falciparum erythrocyte binding antigen-175 (EBA-175) gene in Equatorial Guinea
| العنوان: | Population genetic analysis of the Plasmodium falciparum erythrocyte binding antigen-175 (EBA-175) gene in Equatorial Guinea |
|---|---|
| المؤلفون: | Pei-Kui Yang, Xue-Yan Liang, Min Lin, Jiang-Tao Chen, Hui-Ying Huang, Li-Yun Lin, Carlos Salas Ehapo, Urbano Monsuy Eyi, Yu-Zhong Zheng, Dong-De Xie, Jin-Quan He, Huan-Tong Mo, Xin-Yao Chen, Xiang-Zhi Liu, Ying-E. Wu |
| المصدر: | Malaria Journal, Vol 20, Iss 1, Pp 1-16 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Bioko Island, Bata district, Plasmodium falciparum, PfEBA-175, Natural selection, Genetic diversity, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Abstract Background Plasmodium falciparum erythrocyte binding antigen-175 (PfEBA-175) is a candidate antigen for a blood-stage malaria vaccine, while various polymorphisms and dimorphism have prevented to development of effective vaccines based on this gene. This study aimed to investigate the dimorphism of PfEBA-175 on both the Bioko Island and continent of Equatorial Guinea, as well as the genetic polymorphism and natural selection of global PfEBA-175. Methods The allelic dimorphism of PfEBA-175 region II of 297 bloods samples from Equatorial Guinea in 2018 and 2019 were investigated by nested polymerase chain reaction and sequencing. Polymorphic characteristics and the effect of natural selection were analyzed using MEGA 7.0, DnaSP 6.0 and PopART programs. Protein function prediction of new amino acid mutation sites was performed using PolyPhen-2 and Foldx program. Results Both Bioko Island and Bata district populations, the frequency of the F-fragment was higher than that of the C-fragment of PfEBA-175 gene. The PfEBA-175 of Bioko Island and Bata district isolates showed a high degree of genetic variability and heterogeneity, with π values of 0.00407 & 0.00411 and Hd values of 0.958 & 0.976 for nucleotide diversity, respectively. The values of Tajima's D of PfEBA-175 on Bata district and Bioko Island were 0.56395 and − 0.27018, respectively. Globally, PfEBA-175 isolates from Asia were more diverse than those from Africa and South America, and genetic differentiation quantified by the fixation index between Asian and South American countries populations was significant (FST > 0.15, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1475-2875 |
| Relation: | https://doaj.org/toc/1475-2875 |
| DOI: | 10.1186/s12936-021-03904-x |
| URL الوصول: | https://doaj.org/article/a929e4d3f0dd40478f4715b59ec4c35e |
| رقم الأكسشن: | edsdoj.929e4d3f0dd40478f4715b59ec4c35e |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14752875 |
|---|---|
| DOI: | 10.1186/s12936-021-03904-x |