دورية أكاديمية
Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells
| العنوان: | Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells |
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| المؤلفون: | Guizhi Meng, Hongjuan Duan, Jingying Jia, Baobao Liu, Yun Ma, Xiaoyan Cai |
| المصدر: | Animal Bioscience, Vol 37, Iss 3, Pp 509-521 (2024) |
| بيانات النشر: | Asian-Australasian Association of Animal Production Societies, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Zoology |
| مصطلحات موضوعية: | alfalfa xenomir-162, bovine mammary epithelial cells, mtor pathway, milk protein, proliferation, Zoology, QL1-991 |
| الوصف: | Objective It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the regulatory roles of alfalfa xeno-miR162 on casein synthesis in bovine mammary epithelial cells (bMECs). Methods The effects of alfalfa xenomiR-162 and G protein subunit gamma 11 (GNG11) on proliferation and milk protein metabolism of bMECs were detected by 5-Ethynyl-2′-Deoxyuridine (EdU) staining, flow cytometry, cell counting kit-8 (CCK-8), enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Dual-luciferase reporter assay was used to verify the targeting relationship between GNG11 and xenomiR-162. Results Results showed that over-expression of xenomiR-162 inhibited cell proliferation but promoted apoptosis, which also up-regulated the expression of several casein coding genes, including CSN1S1, CSN1S2, and CSN3, while decreasing the expression of CSN2. Furthermore, the targeting relationship between GNG11 and xenomiR-162 was determined, and it was confirmed that GNG11 silencing also inhibited cell proliferation but promoted apoptosis and reduced the expression of casein coding genes and genes related to the mammalian target of rapamycin (mTOR) pathway. Conclusion Alfalfa xenomiR-162 appears to regulate bMECs proliferation and milk protein synthesis via GNG11 in the mTOR pathway, suggesting that this xeno-miRNA could be harnessed to modulate CSN3 expression in dairy cows, and increase κ-casein contents in milk. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2765-0189 2765-0235 |
| Relation: | http://www.animbiosci.org/upload/pdf/ab-23-0370.pdf; https://doaj.org/toc/2765-0189; https://doaj.org/toc/2765-0235 |
| DOI: | 10.5713/ab.23.0370 |
| URL الوصول: | https://doaj.org/article/c92e7f352c8545e5aff49c25aa4246e6 |
| رقم الأكسشن: | edsdoj.92e7f352c8545e5aff49c25aa4246e6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 27650189 27650235 |
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| DOI: | 10.5713/ab.23.0370 |