دورية أكاديمية
Angiopoietin-1 and Angiopoietin-2 Expression Imbalance Influence in Early Period After Subarachnoid Hemorrhage
| العنوان: | Angiopoietin-1 and Angiopoietin-2 Expression Imbalance Influence in Early Period After Subarachnoid Hemorrhage |
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| المؤلفون: | Hua Gu, Zhen-Hai Fei, Yi-Qi Wang, Jian-Guo Yang, Chao-Hui Zhao, Yong Cai, Xing-Ming Zhong |
| المصدر: | International Neurourology Journal, Vol 20, Iss 4, Pp 288-295 (2016) |
| بيانات النشر: | Korean Continence Society, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | Angiopoietin-1, Angiopoietin-2, Subarachnoid Hemorrhage, Blood-Brain Barrier, Brain Injuries, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Purpose Microvascular endothelial integrity is important for maintaining the blood-brain barrier (BBB). However, subarachnoid hemorrhage (SAH) disrupts this integrity, making the BBB dysfunctional—an important pathophysiological change after SAH. Angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) regulate microvascular permeability by balancing each other’s expression. Methods This study investigated the dynamics of Ang-1 and Ang-2 expression after SAH and the protective effect of Ang-1 on BBB functioning using an endovascular puncture model of rat SAH. The Ang-1 and Ang-2 expression in brain tissue was determined by immunohistochemistry. In addition, Western blotting was used to estimate Ang-1 and Ang-2 concentration and to compare them at 6–72 hours post-SAH cortex and hippocampus. Evans blue viability assay was used to evaluate BBB permeability, and neurological testing was implemented to evaluate neurological impairment during SAH. Results It was found that following SAH, Ang-1 expression decreases and Ang-2 expression increases in the cortex, hippocampus, and microvessels. The Ang-1/Ang-2 ratio decreased as quickly as 6 hours after SAH and reached its lowest 1 day after SAH. Finally, it was found that exogenous Ang-1 reduces SAH-associated BBB leakage and improves neurological function in post-SAH rats. Conclusions Our findings suggest that the equilibrium between Ang-1 and Ang-2 is broken in a period shortly after SAH, and the treatment of exogenous Ang-1 injection alleviates neurological dysfunctions through decreasing BBB destruction. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2093-4777 2093-6931 |
| Relation: | http://www.einj.org/upload/pdf/inj-1632692-346.pdf; https://doaj.org/toc/2093-4777; https://doaj.org/toc/2093-6931 |
| DOI: | 10.5213/inj.1632692.346 |
| URL الوصول: | https://doaj.org/article/937bce03f5b9401183e33e37af083f0b |
| رقم الأكسشن: | edsdoj.937bce03f5b9401183e33e37af083f0b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20934777 20936931 |
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| DOI: | 10.5213/inj.1632692.346 |