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Highly sensitive near-infrared SERS nanoprobes for in vivo imaging using gold-assembled silica nanoparticles with controllable nanogaps

التفاصيل البيبلوغرافية
العنوان: Highly sensitive near-infrared SERS nanoprobes for in vivo imaging using gold-assembled silica nanoparticles with controllable nanogaps
المؤلفون: Sungje Bock, Yun-Sik Choi, Minhee Kim, Yewon Yun, Xuan-Hung Pham, Jaehi Kim, Bomi Seong, Wooyeon Kim, Ahla Jo, Kyeong-Min Ham, Sung Gun Lee, Sang Hun Lee, Homan Kang, Hak Soo Choi, Dae Hong Jeong, Hyejin Chang, Dong-Eun Kim, Bong-Hyun Jun
المصدر: Journal of Nanobiotechnology, Vol 20, Iss 1, Pp 1-12 (2022)
بيانات النشر: BMC, 2022.
سنة النشر: 2022
المجموعة: LCC:Biotechnology
LCC:Medical technology
مصطلحات موضوعية: Surface-enhanced Raman spectroscopy (SERS), Gold nanoparticle, Nanogap, Hotspot, In vivo imaging, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5
الوصف: Abstract Background To take advantages, such as multiplex capacity, non-photobleaching property, and high sensitivity, of surface-enhanced Raman scattering (SERS)-based in vivo imaging, development of highly enhanced SERS nanoprobes in near-infrared (NIR) region is needed. A well-controlled morphology and biocompatibility are essential features of NIR SERS nanoprobes. Gold (Au)-assembled nanostructures with controllable nanogaps with highly enhanced SERS signals within multiple hotspots could be a breakthrough. Results Au-assembled silica (SiO2) nanoparticles (NPs) (SiO2@Au@Au NPs) as NIR SERS nanoprobes are synthesized using the seed-mediated growth method. SiO2@Au@Au NPs using six different sizes of Au NPs (SiO2@Au@Au50–SiO2@Au@Au500) were prepared by controlling the concentration of Au precursor in the growth step. The nanogaps between Au NPs on the SiO2 surface could be controlled from 4.16 to 0.98 nm by adjusting the concentration of Au precursor (hence increasing Au NP sizes), which resulted in the formation of effective SERS hotspots. SiO2@Au@Au500 NPs with a 0.98-nm gap showed a high SERS enhancement factor of approximately 3.8 × 106 under 785-nm photoexcitation. SiO2@Au@Au500 nanoprobes showed detectable in vivo SERS signals at a concentration of 16 μg/mL in animal tissue specimen at a depth of 7 mm. SiO2@Au@Au500 NPs with 14 different Raman label compounds exhibited distinct SERS signals upon subcutaneous injection into nude mice. Conclusions SiO2@Au@Au NPs showed high potential for in vivo applications as multiplex nanoprobes with high SERS sensitivity in the NIR region. Graphical Abstract
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1477-3155
Relation: https://doaj.org/toc/1477-3155
DOI: 10.1186/s12951-022-01327-7
URL الوصول: https://doaj.org/article/c938981375934436818381b4ca3fa611
رقم الأكسشن: edsdoj.938981375934436818381b4ca3fa611
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14773155
DOI:10.1186/s12951-022-01327-7