دورية أكاديمية
Day-to-day and within-day biological variation of cell-free DNAResearch in context
العنوان: | Day-to-day and within-day biological variation of cell-free DNAResearch in context |
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المؤلفون: | Anne Tranberg Madsen, Johanne Andersen Hojbjerg, Boe Sandahl Sorensen, Anne Winther-Larsen |
المصدر: | EBioMedicine, Vol 49, Iss , Pp 284-290 (2019) |
بيانات النشر: | Elsevier, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Medicine LCC:Medicine (General) |
مصطلحات موضوعية: | Medicine, Medicine (General), R5-920 |
الوصف: | Background: Numerous studies have shown that cell-free DNA (cfDNA) levels may serve as a non-invasive biomarker of a broad spectrum of acute and chronic pathologies. However, in order to make clinical decisions based on cfDNA measurements, it is essential to understand the magnitude of biological variation so this variation is not confused with a variation that actually represent a clinically relevant change. The present study was designed to evaluate the biological variation of cfDNA in healthy subjects and lung cancer patients. Methods: Plasma samples were collected from 33 healthy subjects and ten lung cancer patients over three days, as well as during the same day. CfDNA was quantified using droplet digital PCR. Biological variation data was estimated using mixed models. Findings: The within-subject variation was 25% and the between-subject variation was 30%. The reference change value for the healthy subjects was 70%. There was no systematic difference in cfDNA levels from day-to-day (p = 0⋅61), but there was a significant decline during the day (p0⋅3). Interpretation: Our findings show that cfDNA levels fluctuate significantly during the day and exhibit considerable within-subject variation. Thus, the data presented offer a substantial contribution to the interpretation of the clinical significance of cfDNA. Funding: Læge Sofus Carl Emil Friis og hustru Olga Doris Friis' Legat, Harboefonden, and Dagmar Marshalls Fond. Keywords: CfDNA, Biological variation, Biomarker, Monitoring, Droplet digital PCR, Cancer |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2352-3964 |
Relation: | http://www.sciencedirect.com/science/article/pii/S2352396419306723; https://doaj.org/toc/2352-3964 |
DOI: | 10.1016/j.ebiom.2019.10.008 |
URL الوصول: | https://doaj.org/article/9426b61909b04628aad5f225c53004e7 |
رقم الأكسشن: | edsdoj.9426b61909b04628aad5f225c53004e7 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 23523964 |
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DOI: | 10.1016/j.ebiom.2019.10.008 |