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Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil)

التفاصيل البيبلوغرافية
العنوان: Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil)
المؤلفون: C. F. L. Sodré, W. Macedo, L. M. Feitosa, N. S. M. Sousa, R. N. F. Carvalho-Neta, L. F. Carvalho Costa, J. L. S. Nunes, L. Tchaicka
المصدر: Brazilian Journal of Biology, Vol 84 (2024)
بيانات النشر: Instituto Internacional de Ecologia, 2024.
سنة النشر: 2024
المجموعة: LCC:Science
LCC:Biology (General)
LCC:Zoology
LCC:Botany
مصطلحات موضوعية: Elasmobranchii, Sphyrnidae, COI, ITS2, forensic identification, Science, Biology (General), QH301-705.5, Zoology, QL1-991, Botany, QK1-989
الوصف: Abstract Sharks of the genus Sphyrna are under intense exploitation globally. In Brazil’s northern coast, this genus represents a high proportion of fisheries landings and comprises four species. However, due to difficulty of specific identification when specimens are landed, most of the records are limited to the genus level. Here we analyzed the effectiveness of ITS2 (Internal Transcribed Spacer 2 of rDNA) fragment length protocol (Abercrombie et al., 2005) for identifying hammerhead shark species, comparing with the analysis of COI (Cytochrome oxidase subunit I) and ITS2 sequences. We evaluated samples of muscle tissue acquired in the main fishing ports of Maranhão: Carutapera, Raposa e Tutóia. Sampling was conducted between March 2017 to March 2018 and complemented with material deposited in collection (2015). COI results indicated the occurrence of endangered species which are prohibited to be landed. These include Sphyrna mokarran (67%), S. lewini (15%), S. tudes (3%), and S. tiburo (15%). For the ITS2 marker, we investigated the optimization of the protocol developed by Abercrombie (2005) for to improve the use in this geographical area througout design of a new primers.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1678-4375
1519-6984
Relation: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101111&lng=en&tlng=en; http://www.scielo.br/pdf/bjb/v84/1519-6984-bjb-84-e274862.pdf; https://doaj.org/toc/1678-4375
DOI: 10.1590/1519-6984.274862
URL الوصول: https://doaj.org/article/94c2a709d95a41be88ce4898dccd3afe
رقم الأكسشن: edsdoj.94c2a709d95a41be88ce4898dccd3afe
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16784375
15196984
DOI:10.1590/1519-6984.274862