دورية أكاديمية
Changing colors and understanding: the use of mutant chromogenic protein and informational suppressor strains of Escherichia coli to explore the central dogma of molecular biology
| العنوان: | Changing colors and understanding: the use of mutant chromogenic protein and informational suppressor strains of Escherichia coli to explore the central dogma of molecular biology |
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| المؤلفون: | Sarah DeWolf, Maartje Van den Bogaard, Rachael Brady Hart, Sparrow Hartman, Nancy Boury, Gregory J. Phillips |
| المصدر: | Journal of Microbiology & Biology Education, Vol 24, Iss 3 (2023) |
| بيانات النشر: | American Society for Microbiology, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Special aspects of education LCC:Biology (General) |
| مصطلحات موضوعية: | genetics instruction, mutations, central dogma, chromogenic bacteria, Special aspects of education, LC8-6691, Biology (General), QH301-705.5 |
| الوصف: | ABSTRACTThe central dogma of molecular biology is a key concept for undergraduate students in the life sciences as it describes the flow of information in living systems from gene-to-gene product. However, despite often being covered in many introductory life science courses, students may still have misconceptions surrounding the central dogma even as they move on to advanced courses. Active learning strategies such as laboratory activities can be useful in addressing such misconceptions. In the laboratory exercise presented here, senior undergraduate students explore the intricacies of nonsense suppressor mutations to challenge their understanding of the central dogma. The students introduce a plasmid carrying a nonfunctional chromogenic protein gene due to a nonsense mutation in a codon encoding the chromophore to various nonsense suppressor strains of Escherichia coli. Students then observe distinct chromogenic phenotypes, depending on the suppressor strain. Students showed a moderate increase in understanding of the central dogma. While the central dogma remains a challenging concept, active learning strategies like the one presented here can help reduce conceptual errors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1935-7885 1935-7877 |
| Relation: | https://doaj.org/toc/1935-7877; https://doaj.org/toc/1935-7885 |
| DOI: | 10.1128/jmbe.00094-23 |
| URL الوصول: | https://doaj.org/article/9558d1c2bfeb4c1aab91101d05480d7e |
| رقم الأكسشن: | edsdoj.9558d1c2bfeb4c1aab91101d05480d7e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19357885 19357877 |
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| DOI: | 10.1128/jmbe.00094-23 |