دورية أكاديمية
Data on Connexin 43 hemichannels regulation of cellular redox in lens
| العنوان: | Data on Connexin 43 hemichannels regulation of cellular redox in lens |
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| المؤلفون: | Yumeng Quan, Yu Du, Changrui Wu, Sumin Gu, Jean X. Jiang |
| المصدر: | Data in Brief, Vol 39, Iss , Pp 107572- (2021) |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Computer applications to medicine. Medical informatics LCC:Science (General) |
| مصطلحات موضوعية: | Connexin, Hemichannel, Redox metabolite, Redox regulation, Lens, Computer applications to medicine. Medical informatics, R858-859.7, Science (General), Q1-390 |
| الوصف: | This article describes a dataset that is related to the research paper “Connexin hemichannels regulate redox potential via metabolite exchange and protect lens against cellular oxidative damage”. Growing evidence demonstrates that oxidative stress is a key event in cataract formation. Hemichannels (HCs) formed by Connexin (Cx) 43, a Cx subtype only present in the epithelium of lens tissue, mediate the exchange of small molecules between the intracellular and extracellular environments, including redox-related metabolic molecules, such as glutathione (GSH) and reactive oxygen species (ROS). Here, we used a Cx43 heterozygous mouse model, Cx43E2 antibody (a specific Cx43 HC blocker), and knocked down Cx43 expression by siRNA in human lens epithelial HLE-B3 cells to assess the oxidative response of Cx43 HCs to H2O2 and UVB radiation. Western blot analysis of heterozygous Cx43-null (Cx43+/−) mouse lenses showed the haploinsufficiency of Cx43 protein. We further assessed anti-oxidative gene expression in response to H2O2 and UVB radiation treatment in the Cx43-deficient lens epithelial cells. This dataset will be useful for understanding the critical role of Cx43 HCs in maintaining redox homeostasis in the lens under oxidative stress. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3409 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352340921008477; https://doaj.org/toc/2352-3409 |
| DOI: | 10.1016/j.dib.2021.107572 |
| URL الوصول: | https://doaj.org/article/cdea95d1b9af453aa859fa0d513fd49b |
| رقم الأكسشن: | edsdoj.95d1b9af453aa859fa0d513fd49b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523409 |
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| DOI: | 10.1016/j.dib.2021.107572 |