دورية أكاديمية
Synergistic production of 20(S)-protopanaxadiol from protopanaxadiol-type ginsenosides by β-glycosidases from Dictyoglomus turgidum and Caldicellulosiruptor bescii
| العنوان: | Synergistic production of 20(S)-protopanaxadiol from protopanaxadiol-type ginsenosides by β-glycosidases from Dictyoglomus turgidum and Caldicellulosiruptor bescii |
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| المؤلفون: | Ji-Hyeon Choi, Min-Ju Seo, Kyung-Chul Shin, Ki Won Lee, Deok-Kun Oh |
| المصدر: | AMB Express, Vol 7, Iss 1, Pp 1-9 (2017) |
| بيانات النشر: | SpringerOpen, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | α-l-Arabinopyranosidase activity, 20(S)-Protopanaxadiol, β-Glycosidase, Dictyoglomus turgidum, Caldicellulosiruptor bescii, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract 20(S)-Protopanaxadiol (APPD) has potential uses in the pharmaceutical, cosmetic, and food industries because of its anti-stress, anti-fatigue, anti-cancer, anti-inflammatory, and anti-wrinkle properties. However, APPD production is difficult because β-glycosidases that convert the protopanaxadiol (PPD)-type ginsenoside compound K to APPD are rare. β-Glycosidase from Dictyoglomus turgidum (DT-bgl) has the highest specific activity for converting compound K to APPD, but exhibits no activity towards the α-l-arabinopyranoside moiety in compound Y. Therefore, β-glycosidase from Caldicellulosiruptor bescii (CB-bgl), which has a strong α-l-arabinopyranosidase activity, was used along with DT-bgl. The volumetric and specific productivities of the two-enzyme system for APPD using ginseng root extract were 38.4- and 38.7-fold higher, respectively, than those of β-glycosidase from Pyrococcus furiosus, which had the highest volumetric productivity previously reported, at the same enzyme and substrate concentrations. Thus, DT-bgl combined with CB-bgl completely converted PPD-type ginsenosides to APPD with the highest volumetric and specific productivities reported thus far. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | http://link.springer.com/article/10.1186/s13568-017-0524-9; https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-017-0524-9 |
| URL الوصول: | https://doaj.org/article/95e103b9f5c5498ca38504fc96b181ee |
| رقم الأكسشن: | edsdoj.95e103b9f5c5498ca38504fc96b181ee |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-017-0524-9 |