دورية أكاديمية

First Report on Leptospira Species Isolated from Patients in Slovenia

التفاصيل البيبلوغرافية
العنوان: First Report on Leptospira Species Isolated from Patients in Slovenia
المؤلفون: Eva Ružić-Sabljić, Daša Podgoršek, Katja Strašek Smrdel, Andraž Celar Šturm, Mateja Logar, Andrea Pavlović, Tatjana Remec, Zvonko Baklan, Emil Pal, Tjaša Cerar Kišek
المصدر: Microorganisms, Vol 11, Iss 11, p 2739 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: Leptospira sp., leptospirosis, Slovenia, lfb gene, MALDI-TOF, NotI-RFLP, Biology (General), QH301-705.5
الوصف: Leptospirosis is an important worldwide zoonosis, and it has also been reported in Slovenia. The cultivation of Leptospira from human material is difficult. Despite that, we successfully isolated 12 human Leptospira strains isolated from patients between 2002 and 2020 and used various methods for the phenotypic and genotypic characterization of the strains, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) using our own MALDI-TOF data library, melting temperature analysis of the amplified lfb1 gene, determination of Leptospira serogroups using rabbit immune sera, NotI-RFLP of the whole Leptospira genome, multilocus sequence typing (MLST) of seven housekeeping genes, and whole-genome sequencing (WGS)-based typing. We confirmed the presence of four pathogenic Leptospira species (L. kirschneri, L. interrogans, L. borgpetersenii, and L. santarosai) and three serogroups: Grippotyphosa, Icterohaemorrhagiae, and Sejroe. MALDI-TOF identified three of seven isolates at the species level and four isolates at the genus level. Serovars of 8 of the 10 strains were determined using NotI-RFLP. MLST showed that the clinical isolates belonged to sequence types ST17, ST110, and ST155. WGS confirmed the analysis of Leptospira strains using conventional methods. In addition, WGS provided better taxonomic resolution for isolate DDA 10944/10.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-2607
Relation: https://www.mdpi.com/2076-2607/11/11/2739; https://doaj.org/toc/2076-2607
DOI: 10.3390/microorganisms11112739
URL الوصول: https://doaj.org/article/96228c545eea4d99998695b3d1f75c6e
رقم الأكسشن: edsdoj.96228c545eea4d99998695b3d1f75c6e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20762607
DOI:10.3390/microorganisms11112739