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Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4

التفاصيل البيبلوغرافية
العنوان: Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4
المؤلفون: John M Nicoludis, Bennett E Vogt, Anna G Green, Charlotta PI Schärfe, Debora S Marks, Rachelle Gaudet
المصدر: eLife, Vol 5 (2016)
بيانات النشر: eLife Sciences Publications Ltd, 2016.
سنة النشر: 2016
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: protocadherins, cell adhesion, sequence coevolution, crystallography, Medicine, Science, Biology (General), QH301-705.5
الوصف: Protocadherins (Pcdhs) are cell adhesion and signaling proteins used by neurons to develop and maintain neuronal networks, relying on trans homophilic interactions between their extracellular cadherin (EC) repeat domains. We present the structure of the antiparallel EC1-4 homodimer of human PcdhγB3, a member of the γ subfamily of clustered Pcdhs. Structure and sequence comparisons of α, β, and γ clustered Pcdh isoforms illustrate that subfamilies encode specificity in distinct ways through diversification of loop region structure and composition in EC2 and EC3, which contains isoform-specific conservation of primarily polar residues. In contrast, the EC1/EC4 interface comprises hydrophobic interactions that provide non-selective dimerization affinity. Using sequence coevolution analysis, we found evidence for a similar antiparallel EC1-4 interaction in non-clustered Pcdh families. We thus deduce that the EC1-4 antiparallel homodimer is a general interaction strategy that evolved before the divergence of these distinct protocadherin families.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/18449; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.18449
URL الوصول: https://doaj.org/article/969b0c85b4b5405ea48a896dbd59b91d
رقم الأكسشن: edsdoj.969b0c85b4b5405ea48a896dbd59b91d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.18449