دورية أكاديمية
Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency
| العنوان: | Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency |
|---|---|
| المؤلفون: | Byoung-San Moon, David Huang, Fan Gao, Mingyang Cai, Guochang Lyu, Lei Zhang, Jun Chen, Wange Lu |
| المصدر: | Cell Death Discovery, Vol 9, Iss 1, Pp 1-13 (2023) |
| بيانات النشر: | Nature Publishing Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Cytology |
| مصطلحات موضوعية: | Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671 |
| الوصف: | Abstract Nuclear architecture underlies the transcriptional programs within the cell to establish cell identity. As previously demonstrated, long-range chromatin interactions of the Oct4 distal enhancer (DE) are correlated with active transcription in naïve state embryonic stem cells. Here, we identify and characterize extreme long-range interactions of the Oct4 DE through a novel CRISPR labeling technique we developed and chromosome conformation capture to identify lethal giant larvae 2 (Llgl2) and growth factor receptor-bound protein 7 (Grb7) as putative functional interacting target genes in different chromosomes. We show that the Oct4 DE directly regulates expression of Llgl2 and Grb7 in addition to Oct4. Expression of Llgl2 and Grb7 closely correlates with the pluripotent state, where knock down of either result in loss of pluripotency, and overexpression enhances somatic cell reprogramming. We demonstrated that biologically important interactions of the Oct4 DE can occur at extreme distances that are necessary for the maintenance of the pluripotent state. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2058-7716 |
| Relation: | https://doaj.org/toc/2058-7716 |
| DOI: | 10.1038/s41420-023-01363-8 |
| URL الوصول: | https://doaj.org/article/ee96c1692bd0487298c7416fff3bd225 |
| رقم الأكسشن: | edsdoj.96c1692bd0487298c7416fff3bd225 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20587716 |
|---|---|
| DOI: | 10.1038/s41420-023-01363-8 |