دورية أكاديمية
High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris
| العنوان: | High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris |
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| المؤلفون: | Zhi-Xiang Xiang, Jin-Song Gong, Jin-Hao Shi, Chun-Fang Liu, Heng Li, Chang Su, Min Jiang, Zheng-Hong Xu, Jin-Song Shi |
| المصدر: | Bioresources and Bioprocessing, Vol 9, Iss 1, Pp 1-13 (2022) |
| بيانات النشر: | SpringerOpen, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Technology LCC:Chemical technology LCC:Biotechnology |
| مصطلحات موضوعية: | Human-like collagen, Secretory expression, Fermentation, Protein purification, Characterization, Technology, Chemical technology, TP1-1185, Biotechnology, TP248.13-248.65 |
| الوصف: | Abstract Collagen, the highest content protein in the body, has irreplaceable biological functions, and it is widespread concerned in food, beauty, and medicine with great market demand. The gene encoding the recombinant type III human-like collagen α1 chain fragment was integrated into P. pastoris genome after partial amino acids were substituted. Combined with promoter engineering and high-density fermentation technology, soluble secretory expression with the highest yield of 1.05 g L−1 was achieved using two-stage feeding method, and the purity could reach 96% after affinity purification. The determination of N/C-terminal protein sequence were consistent with the theoretical expectation and showed the characteristics of Gly-X-Y repeated short peptide sequence. In amino acid analysis, glycine shared 27.02% and proline 23.92%, which were in accordance with the characteristics of collagen. Ultraviolet spectrum combined with Fourier transform infrared spectroscopy as well as mass spectrometry demonstrated that the target product conformed to the characteristics of collagen spectrums and existed as homologous dimer and trimer in the broth. This work provided a sustainable and economically viable source of the recombinant type III human-like collagen. Graphical Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2197-4365 |
| Relation: | https://doaj.org/toc/2197-4365 |
| DOI: | 10.1186/s40643-022-00605-4 |
| URL الوصول: | https://doaj.org/article/cac96d6eb8cc40fc9a67d567cf90d99c |
| رقم الأكسشن: | edsdoj.96d6eb8cc40fc9a67d567cf90d99c |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21974365 |
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| DOI: | 10.1186/s40643-022-00605-4 |