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Structural Basis for the Mechanism of ATP-Dependent Acetone Carboxylation

التفاصيل البيبلوغرافية
العنوان: Structural Basis for the Mechanism of ATP-Dependent Acetone Carboxylation
المؤلفون: Florence Mus, Brian J. Eilers, Alexander B. Alleman, Burak V. Kabasakal, Jennifer N. Wells, James W. Murray, Boguslaw P. Nocek, Jennifer L. DuBois, John W. Peters
المصدر: Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
بيانات النشر: Nature Portfolio, 2017.
سنة النشر: 2017
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract Microorganisms use carboxylase enzymes to form new carbon-carbon bonds by introducing carbon dioxide gas (CO2) or its hydrated form, bicarbonate (HCO3 −), into target molecules. Acetone carboxylases (ACs) catalyze the conversion of substrates acetone and HCO3 − to form the product acetoacetate. Many bicarbonate-incorporating carboxylases rely on the organic cofactor biotin for the activation of bicarbonate. ACs contain metal ions but not organic cofactors, and use ATP to activate substrates through phosphorylation. How the enzyme coordinates these phosphorylation events and new C-C bond formation in the absence of biotin has remained a mystery since these enzymes were discovered. The first structural rationale for acetone carboxylation is presented here, focusing on the 360 kDa (αβγ)2 heterohexameric AC from Xanthobacter autotrophicus in the ligand-free, AMP-bound, and acetate coordinated states. These structures suggest successive steps in a catalytic cycle revealing that AC undergoes large conformational changes coupled to substrate activation by ATP to perform C-C bond ligation at a distant Mn center. These results illustrate a new chemical strategy for the conversion of CO2 into biomass, a process of great significance to the global carbon cycle.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-017-06973-8
URL الوصول: https://doaj.org/article/974d0ab5c0a34d4b9a8c4a31370e6562
رقم الأكسشن: edsdoj.974d0ab5c0a34d4b9a8c4a31370e6562
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-017-06973-8