دورية أكاديمية
Biological Activity of Picrorhiza kurroa: A Source of Potential Antimicrobial Compounds against Yersinia enterocolitica
العنوان: | Biological Activity of Picrorhiza kurroa: A Source of Potential Antimicrobial Compounds against Yersinia enterocolitica |
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المؤلفون: | Anju Thapa, Ravinder Kaushik, Smriti Arora, Sundeep Jaglan, Varun Jaswal, Virendra Kumar Yadav, Manjeet Singh, Aarti Bains, Prince Chawla, Azhar Khan, Melinda Fogarasi, Szabolcs Fogarasi |
المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 22, p 14090 (2022) |
بيانات النشر: | MDPI AG, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | P. kurroa, antimicrobial potential, picroside–1, Yersinia enterocolitica, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | Yersiniosis, caused by Yersinia enterocolitica, is the third most rampant zoonotic disease in Europe; the pathogen shows high antibiotic resistance. Herbs have multiple anti–microbial components that reduce microorganism resistance. Therefore, an extract of Picrorhiza kurroa (P. kurroa) was evaluated for potential antimicrobial activity. We report that the ethanolic extract of P. kurroa showed effective antimicrobial activity (zone of inhibition: 29.8 mm, Minimum inhibitory concentration (MIC): 2.45 mg/mL, minimum bactericidal concentration (MBC): 2.4 mg/mL) against Yersinia enterocolitica. Potential bioactive compounds from P. kurroa were identified using LC–MS, namely, cerberidol, annonidine A, benzyl formate, picroside–1, and furcatoside A. P. kurroa showed effective antimicrobial potential in skim milk at different pH, acidity, and water activity levels. P. kurroa affected the physiology of Yersinia enterocolitica and reduced the number of live cells. Yersinia enterocolitica, when incubated with P. kurroa extract, showed lower toxin production. Picroside–1 was isolated and showed higher antimicrobial potential in comparison to the standard antibiotic. Picroside–1 lysed the Yersinia enterocolitica cells, as observed under scanning electron microscopy. Docking revealed that picroside–1 (ligand) showed both hydrophilic and hydrophobic interactions with the dihydrofolate reductase (DHFR) protein of Yersinia enterocolitica and that DHFR is a possible drug target. The high activity and natural origin of Picroside–1 justify its potential as a possible drug candidate for Yersinia enterocolitica. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 1661-6596 |
Relation: | https://www.mdpi.com/1422-0067/23/22/14090; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms232214090 |
URL الوصول: | https://doaj.org/article/989701376a874ada9919d29c6aa15745 |
رقم الأكسشن: | edsdoj.989701376a874ada9919d29c6aa15745 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14220067 16616596 |
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DOI: | 10.3390/ijms232214090 |