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Detection of Pathogenic E. Coli by Electrochemical Biosensors Based on Aptamers Designed by Bioinformatic Tools

التفاصيل البيبلوغرافية
العنوان: Detection of Pathogenic E. Coli by Electrochemical Biosensors Based on Aptamers Designed by Bioinformatic Tools
المؤلفون: Jose L. Ropero-Vega, Leidy J. Albiares-Sánchez, Wendy R. Leon-Sanchez, Wilfredo Valdivieso-Quintero, Johanna M. Florez-Castillo
المصدر: Chemical Engineering Transactions, Vol 93 (2022)
بيانات النشر: AIDIC Servizi S.r.l., 2022.
سنة النشر: 2022
المجموعة: LCC:Chemical engineering
LCC:Computer engineering. Computer hardware
مصطلحات موضوعية: Chemical engineering, TP155-156, Computer engineering. Computer hardware, TK7885-7895
الوصف: In this work, we present the in silico design of a new aptamer (named Apt917) capable of interacting with E. coli and its evaluation as a recognition element in electrochemical biosensors. A search and bioinformatic analysis of various aptamers reported in the literature was carried out. Parameters such as low dissociation constant and short sequences were considered to design and model new hybrid sequences. The designed Apt917 has a minimum free energy and high percent to frequency, making it promising compared to the initial ones. Apt917 was synthesized and immobilized on gold nanoparticles modified screen printed electrodes. The evaluation of the obtained biosensor shows a promising detection of E. coli O157:H7 with limits of detection and quantification of 8 and 27 CFU/mL, respectively.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2283-9216
Relation: https://www.cetjournal.it/index.php/cet/article/view/12545; https://doaj.org/toc/2283-9216
DOI: 10.3303/CET2293048
URL الوصول: https://doaj.org/article/9935f4cfc46f4460b05f028684c9e066
رقم الأكسشن: edsdoj.9935f4cfc46f4460b05f028684c9e066
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22839216
DOI:10.3303/CET2293048